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Products_type
Recombinant Protein
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Products_short_name
[CD117]
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Other_names
[chromogranin-A preproprotein; Chromogranin-A; chromogranin-A; SP-I; pituitary secretory protein I; parathyroid secretory protein 1; betagranin (N-terminal fragment of chromogranin A); chromogranin A (parathyroid secretory protein 1); Pituitary secretory protein I; SP-ICleaved into the following 13 chains:Vasostatin-1; Alternative name(s):; Vasostatin IVasostatin-2; Alternative name(s):; Vasostatin II]
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Other_gene_names
[CHGA; CHGA; CGA; CgA; SP-I]
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Purity
Highly Purified~95% (SDS-PAGE, Coomassie Brilliant Blue)
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Form
Supplied as lyophilized powder. Reconstitute with 500ul sterile PBS.
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Storage_stability
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_application
Western Blot (WB)
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Description
For cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
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Gene target
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Gene symbol
KIT
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Short name
CD117, Recombinant, (c-Kit, KIT, Mast/stem Growth Factor Receptor Kit, p145 c-kit, Piebald Trait Protein, PBT, Proto-oncogene c-Kit, SCFR, Tyrosine-protein Kinase Kit, v-kit Hardy-Zuckerman 4 Feline Sarcoma Oncogene Homolog)
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Technique
Recombinant, Feline, felines, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Sometimes til 5% of an area felines have specific IgGs for the feline retroviral feLV infecting Felidae. Also distemper and leukemia virusses apear in cats and have their specific IgG antibodies that are very stable. All cats used for producing immunoglobulins are screened and tested virus negative. Cat seras have usually higher IgG tirters than dog serum. Feline range = 10 to 20 mg/ml IgGs. Humans
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Alternative name
CD117, Rec., H. sapiens (c-reagent, reagent, Mast/progenitor cellular Growth Factor Receptor reagent, p145 c-reagent, Piebald Trait Protein, PBT, Proto-oncogene c-reagent, SCFR, Tyrosine-protein phosphorylation catalyst reagent, v-reagent Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog)
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Alternative technique
rec, cats, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
cell, stem
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Virus
sarcoma
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