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Synonyms
STMN1, Stathmin, C1orf215, LAP18, OP18
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Alternative_names
STMN1, Stathmin, C1orf215, LAP18, OP18
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Description
An important regulatory protein of microtubule dynamics.
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
>92%
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Assay
SDS-PAGE
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Molecular Weight
18.1 kDa
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Storage Temp
+4°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Dry powder
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Physical form description
Lyophilized powder
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Reconstitution Instructions
Reconstitute in sterile PBS, pH7.4 to a stock solution of 400 µg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% (W/V) HSA or BSA) is recommended for further dilution and long term storage.
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Background Information
Stathmin (STMN1), a member of the stathmin family, is also known as Leukemia-associated phosphoprotein p18, Oncoprotein 18 (Op18), Phosphoprotein p19 (pp19), Protein Pr22 and pp17, which contains one SLD (stathmin-like) domain. The function of STMN1 as an important regulatory protein of microtubule dynamics has been well-characterized. Stathmin (STMN1) interacts with two molecules of dimeric α,β-tubulin to form a tight ternary complex called the T2S complex.One mole of STMN1 binds to two moles of tubulin dimers through the stathmin-like domain (SLD).When STMN1 sequesters tubulin into the T2S complex, tubulin becomes non-polymerizable. Without tubulin polymerization, there is no microtubule assembly.STMN1 also promotes microtubule disassembly by acting directly on the microtubule ends.
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Amino acid sequence
Ala 2 - Asp 149
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.