Human Recombinant MDH2
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Catalog number6356-50
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PricePlease ask
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Size50 ug
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SynonymsHuman Recombinant MDH2
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Alternative_namesMalate dehydrogenase 2, NAD (mitochondrial), M-MDH, MDH, MOR1
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DescriptionInvolved in oxidation of malate to OAA
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RecombinantYes
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SourceE. Coli
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Biological activitySpecific activity is > 30 units/mg, and is defined as the amount of enzyme that cleaves 1 µmole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25°C.
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Unit DefinitionSpecific activity is defined as the amount of enzyme that cleaves 1 µmole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25°C.
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Molecular Weight35.2 kDa (335 aa, 25-338 aa + NT His Tag)
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Storage Temp-20°C
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Shippinggel pack
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Shelf Life12 months
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Concentration1 mg/ml
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AppearanceLiquid
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Physical form description1 mg/ml solution in 20 mM Tris-HCl (pH 7.5) containing 10% glycerol.
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Background InformationMDH2 catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. MDH2 is localized to the mitochondria and takes part in the malate-aspartate shuttle that functions in the metabolic coordination between cytosol and mitochondria. MDH2 is highly expressed in the adrenal system, small intestine, heart and pancreas.
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Amino acid sequenceMGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET KAAVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMICVIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL TALTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVKSQET ECTYFSTPLL LGKKGIEKNL GIGKVSSFEE KMISDAIPEL KASIKKGEDF VKTLK
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolMDH2
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Short nameRecombinant MDH2
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameH. sapiens Rec. MDH2
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namemalate dehydrogenase 2
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Synonyms gene name
- malate dehydrogenase 2, NAD (mitochondrial)
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Locus
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Discovery year1986-01-01
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Entrez gene record
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data