Human Recombinant HSC70 Protein

3x Human Recombinant HSC70 Protein found in StressMark proteins

  • Stock availability
    In Stock
  • Scientific context
    HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).
  • Protein target
  • Protein reactivity
  • Certificate of analysis
    This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 3.2µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH 8) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
  • Protein description
    Active Human Recombinant HSC70 Protein
  • Other name
    Constitutive heat shock protein 70 Protein, HSC54 Protein, HSC71 Protein, HSC73 Protein, HSP71 Protein, HSP74 Protein, HSPA10 Protein, HSPA8 Protein, LAP1 Protein, NIP71 Protein
  • Primary research area
    Cancer, Heat Shock
  • Category
  • Brand name
  • Origin
  • NCBI number
  • Gene number
  • Protein number
  • Verified applications
    WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA
  • Relevant bio activity
    ATPase active
  • Protein expression model
    E. coli
  • Protein charasterics
    See included datasheet.
  • Peptide sequence
    See included datasheet.
  • Protein purification
    Affinity Purified
  • Purity pourcentage
    >90% High purity
  • Protein concentration
    Lot/batch specific. See included datasheet.
  • Protein specificity
    ~70 kDa
  • Protein tag
    His tag
  • Storage recommendations
  • Shipping recommendations
    Blue Ice or 4°C
  • Supplementary useful information
    Please see included datasheet or contact us
  • Protein cell localization
    Cytoplasm, Melanosome
  • Bibliography
    1. Brown C. L., et al. (1993) J.Cell Biol. 120(5): 1101-1112 2. Boorstein W. R., Ziegelhoffer T. & Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell. 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294. 6. Brown C. L., et al. (1996) J. Biol. Chem. 271(2): 833-840.
  • Release date
  • PubMed number
    28026090, 26244546, 25939977, 25979775, 23291500, 20084527, 19209902
  • Tested applications
    Western Blot, Functional Assay, Intrinsic fluorescence spectroscopy, Western Blot Control, Functional Assay, Functional Assay, Western Blot Control
  • Tested species reactivity
    Human, Mouse, Photinus pyralis
  • Representative figure legend
    SDS-PAGE of 73kDa Hsc70 protein (SPR-106). SDS-Page of human HSC70 Protein (SPR-106)
  • Warnings
    Non-hazardous materials
  • Protein origin
  • Total weight (kg)
  • Gene target
  • Short name
    Recombinant HSC70 Protein
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. StressMark proteins advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    H. sapiens recombinant HSC70 Protein
  • Alternative technique

SPR-106C | Human Recombinant HSC70 Proteinsize: 2x100 µg | 661.77 USD

Ajax processing

SPR-106A | Human Recombinant HSC70 Proteinsize: 50 µg | 279.41 USD

  • Recommended buffer for storage
    50mM Tris/HCl, pH8, 0.3M NaCl
  • Net weight (g)
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Source
    Recombinants or rec. proteins
  • Group
1. Gene info
  • Identity
  • Gene
  • Long gene name
    heat shock protein family A (Hsp70) member 8
  • Synonyms gene
  • Synonyms gene name
    • heat shock 70kD protein 8
    • heat shock 70kDa protein 8
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Spliceosomal P complex
    • Heat shock 70kDa proteins
    • Spliceosomal C complex
  • Havana BLAST/BLAT
MeSH Data
  • Name
  • Concept
    Scope note:The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
Additional information

SPR-106B | Human Recombinant HSC70 Proteinsize: 100 µg | 372.55 USD

Ajax processing
Simillar products
Supplier:MBS Recombinant
Price:2 099.28USD
Supplier:MBS Recombinant
Price:1 922.81USD
Supplier:ABM microrna
Supplier:MBS Recombinant
Price:2 180.16USD
Supplier:BlueGen ELISAs
Supplier:ABM microrna
Supplier:ABM microrna
Price:2 666.69USD
Supplier:ABM CrispR
Price:7 133.64USD
Human Recombinant HSC70 Protein -
Chat with employee