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Synonyms
Human Recombinant GSTM 2
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Alternative_names
Glutathione S-transferase Mu 2, GST4, GSTM, GSTM2-2, GTHMUS.
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Description
Plays a role in detoxification of electrophilic compounds
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Recombinant
Yes
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Source
E. Coli
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
Specific activity is < 25 units/mg.
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Unit Definition
Specific activity is defined as the amount of enzyme that conjugates 1.0 µ mole of 1-chloro-2, 4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C.
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Molecular Weight
27.9 kDa
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Storage Temp
-20°C
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Shipping
gel pack
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Shelf Life
12 months
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Concentration
0.5 mg/ml
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Appearance
Liquid
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Physical form description
0.5 mg/ml solution in 20 mM Tris HCl (pH 8.0) containing 10% glycerol, 0.1 M NaCl and 1 mM DTT.
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Background Information
Glutathione S-transferase Mu 2 (GSTM2) belongs to the Glutathione S-Transferase (GST) family of proteins. There are 8 families of GST proteins: alpha, kappa, mu, omega, pi, sigma, theta and zeta, each of which is composed of proteins that have various functions throughout the cell. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are structured in a gene cluster on chromosome 1p13.3 and are proven to be highly polymorphic. These genetic variants can change an individual's susceptibility to carcinogens and toxins as well as have an effect on the toxicity and efficacy of several drugs.
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Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MPMTLGYWNI RGLAHSIRLL LEYTDSSYEE KKYTMGDAPD YDRSQWLNEK FKLGLDFPNL PYLIDGTHKI TQSNAILRYI ARKHNLCGES EKEQIREDIL ENQFMDSRMQ LAKLCYDPDF EKLKPEYLQA LPEMLKLYSQ FLGKQPWFLG DKITFVDFIA YDVLERNQVF EPSCLDAFPN LKDFISRFEG LEKISAYMKS SRFLPRPVFT KMAVWGNK
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants