Human Recombinant CDC37 Protein

• Catalog number
  SPR-304B
• Price
  Please ask
• Size
  100 µg

• Stock availability
  In Stock
• Scientific context
  HSP90 co-chaperone Cdc37 is a protein that is encoded by the CDC37 gene. It has been found to form complexes with HSP90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK and elF-2 alpha kinases. It is thought to play a critical role in directing HSP90 to its target kinases (1, 2). CDC37 is necessary for maintaining prostate tumor cell growth and represents a novel target in the exploration for multi-targeted therapies (3, 4).
• Protein target
  CDC37
• Protein reactivity
  Human
• Certificate of analysis
  This product has been certified >90% pure using SDS PAGE analysis. 4uM SPR-303, when added to 2uM SPR-300 (Aha1)-activated HSP90 (2uM; His-tagged HSP90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C, eliminated all Aha1-mediated ATPase stimulation as well as intrinsic HSP90 ATPase activity. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).
• Protein description
  Human Recombinant CDC37 Protein
• Primary research area
  Cancer, Heat Shock, Cell Signaling, Epigenetics
• Category
  Protein
• Brand name
  none
• Origin
  Recombinant
• NCBI number
  NP_008996.1
• Gene number
  11140
• Protein number
  Q16543
• Verified applications
  WB, SDS-PAGE, Functional Assay
• Relevant bio activity
  CDC37 Protein
• Protein expression model
  E. coli
• Protein charasterics
  See included datasheet.
• Peptide sequence
  MGHHHHHHMVDYSVWDHIEVSDDEDETHPNIDTASLFRWRHQARVERMEQFQKEKEELDRGCRECKRKVAECQRKLKELEVAEGGKAELERLQAEAQQLRKEERSWEQKLEEMRKKEKSMPWNVDTLSKDGFSKSMVNTKPEKTEEDSEEVREQKHKTFVEKYEKQIKHFGMLRRWDDSQKYLSDNVHLVCEETANYLVIWCIDLEVEEKCALMEQVAHQTIVMQFILELAKSLKVDPRACFRQFFTKIKTADRQYMEGFNDELEAFKERVRGRAKLRIEKAMKEYEEEERKKRLGPGGLDPVEVYESLPEELQKCFDVKDVQMLQDAISKMDPTDAKYHMQRCIDSGLWVPNSKASEAKEGEEAGPGDPLLEAVPKTGDEKDVSV
• Protein purification
  Affinity Purified
• Purity pourcentage
  >90% High purity
• Recommended buffer for storage
  25mM Tris buffer pH7.2, 150mM NaCl, 0.1DTT, 50% glycerol
• Protein concentration
  Lot/batch specific. See included datasheet.
• Protein specificity
  ~445 kDa
• Protein tag
  His tag
• Storage recommendations
  -20°C
• Shipping recommendations
  Blue Ice or 4°C
• Supplementary useful information
  Please see included datasheet or contact us
• Protein cell localization
  Cytoplasm
• Bibliography
  1. Dai K., Kobayashi R., Beach D. (1996) J Biol Chem. 271(36): 22030-22034. 2. Stepanova L., Leng X., Parker S.B., Harper J.W. (1996) Genes Dev. 10(12): 1491-1502. 3. Kimura Y., et al. (1997) Genes Dev. 11(14): 1775-1185. 4. Gray P.J., et al. (2008) Nat Rev Cancer. 8(7): 491-495.
• Release date
  1-Sep-2011
• PubMed number
  Not added. Please refer to PubMed
• Tested applications
  to be tested
• Tested species reactivity
  to be tested
• Representative figure
• Representative figure legend
  Western blot showing pull down data for 44.5 kDa human his-tagged CDC37 with 90kDa Hsp90 (SPR-304). Western Blot of human CDC37 Protein (SPR-304)
• Warnings
  Non-hazardous materials
• Protein origin
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Other related products
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CDC37   Protein  
• Gene symbol
  CDC37
• Short name
  Recombinant CDC37 Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. StressMark proteins advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. cellular division cycle 37 homolog (S. cerevisiae) Protein
• Alternative technique
  rec
• Alternative to gene target
  cell division cycle 37 homolog (S. cerevisiae), P50CDC37, CDC37 and IDBG-27267 and ENSG00000105401 and 11140, Hsp90 protein binding, Cytoplasm, Cdc37 and IDBG-139272 and ENSMUSG00000019471 and 12539, CDC37 and IDBG-643270 and ENSBTAG00000011699 and

Gene info

• Identity
  HGNC:1735
• Gene
  CDC37
• Long gene name
  cell division cycle 37, HSP90 cochaperone
• Synonyms gene name
  • CDC37 (cell division cycle 37, S. cerevisiae, homolog)
  • CDC37 cell division cycle 37 homolog (S. cerevisiae)
  • cell division cycle 37 homolog (S. cerevisiae)
• Synonyms
  • P50CDC37
• Synonyms name
  • CDC37 cell division cycle 37 homolog
  • Hsp90 co-chaperone Cdc37
  • CDC37 (cell division cycle 37, S. cerevisiae, homolog)
• GenBank acession
  • U63131
• Locus
  19p13.2
• Discovery year
  1999-09-07
• Entrez gene record
  11140
• Pubmed identfication
  • 8703009
  • 8666233
• RefSeq identity
  • NM_007065
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000180578

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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