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ELISA s application
For quantitative measurement of mouse Laminin in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
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Gene tested
murine Laminin
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ELISA standard curve
Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.097 + 0.120 + 0.163 + 0.252 + 0.482 + 0.930 + 1.654 + 2.438
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ELISA kit detection limits
156pg/ml to 10000pg/ml
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ELISA s sensitivity
< 10pg/ml
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Target of this ELISA
Natural mouse Laminin
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Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
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ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);1266;3866;6831;1576;4364;7224 ELISA standard deviations 58.24;204.9;457.7;91.41;270.6;534.6 CV(%);4.6;5.3;6.7;5.8;6.2;7.4
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ELISA kit description
GENTAUR's mouse Laminin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibodies from rabbit specific for Laminin was used as capure antibodies on the ELISA test plate. The internal standards (from murine sarcoma basement membrane) and your samples are measured with a biotin conjugated polyclonal from rabbit specific for Laminin will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse Laminin in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
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Detector and capure antibodies
a polyclonal antibodies from rabbit;a biotinylated polyclonal antibodies from rabbit
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The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse Laminin antibodies;1 ST0000-10;Lyophilized mouse Laminin standard;10ng/tubex2 AR1107;Biotinylated anti- mouse Laminin antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
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Anti coagulation
heparin or EDTA
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How the elisa works
Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse Laminin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each mouse Laminin standard solution and each sample be measured in duplicate.
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Minutes of TMB incubation
15-20
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More gene info
Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2. Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology.
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Antigen
from murine sarcoma basement membrane
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Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Uniprot
Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2
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Gene name
laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2(laminin S)
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Alternative
Lamb2;_x000D_LAMB2_HUMAN;_x000D_LAMC1;_x000D__x000D__x000D_LAMC2;_x000D_LAMC3;_x000D_laminin A;_x000D_Laminin B;_x000D_Laminin B1s chain;_x000D_Laminin gamma 1;_x000D_Laminin gamma 2;_x000D_Laminin gamma 3;_x000D_Laminin subunit beta-2;_x000D_Laminin-11 subunit beta;_x000D_Laminin-14 subunit beta;_x000D_Laminin-15 subunit beta;_x000D_Laminin-3 subunit beta;_x000D_Laminin-4 subunit beta;_x000D_Laminin-7 subunit beta;_x000D_Laminin-9 subunit beta;_x000D_OCCM;_x000D_S-LAM beta;_x000D_S-laminin subunit beta
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Gen protein identity
E9PUR4
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Entrez nr
16774
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
LAMA1; LAMA2; LAMB1; LAMB2
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Short name
Mouse Laminin PicoKine ELISA Kit
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Technique
ELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
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Host
mouse, antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Mouse, Mouses
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Alternative name
Mouse Laminin PicoKine Enzyme-linked immunosorbent assay reagent
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Alternative technique
murine, elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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