Human IL7R CD127 PicoKine ELISA Kit
Human IL7R CD127 PicoKine ELISA Kit is available 2 times from Boster labs
- ELISA's applicationFor quantitative measurement of human IL7R in cell culture supernatant and serum.
- Gene testedHuman IL7R
- ELISA standard curveConcentration(pg/ml) + 0 + 312 + 625 + 1250 + 2500 + 5000 + 10000 + 20000 O.D. + 0.037 + 0.158 + 0.285 + 0.502 + 0.855 + 1.315 + 1.796 + 2.299
- ELISA kit detection limits312pg/ml to 20000pg/ml
- ELISA's sensitivity< 10pg/ml
- Target of this ELISANatural and recombinant human IL7R
- Cross reactionthe ELISA does not cross react with any other proteins except from closely related species
- ELISA's precision parametersintra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);2.8;8;14.3;3.4;9.1;16.2 ELISA standard deviations 0.168;0.36;0.815;0.245;0.51;1.07 CV(%);6;4.5;5.7;7.2;5.6;6.6
- ELISA kit descriptionGENTAUR's human IL7R ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for IL7R was used as capure antibodies on the ELISA test plate. The internal standards (NSO, E21-I262) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL7R will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human IL7R in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
- Detector and capure antibodiesa monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
- The ELISA containsCatalog number;Description;Quantity -;96-well plate precoated with anti- human IL7R antibodies;1 ST0000-20;Lyophilized recombinant human IL7R standard;20ng/tube×2 AR1107;Biotinylated anti- human IL7R antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
- How the elisa worksSample the amount of 100ul/well of the standard solution at 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human IL7R standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant or serum to the ELISA plate. Keep in mind that each human IL7R standard solution and each sample be measured in duplicate.
- Minutes of TMB incubation20-25
- More gene infoThe interleukin-7 receptor(IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7(IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain(IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V(D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.
- AntigenNSO, E21-I262
- Keep atStore at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
- UniprotInterleukin-7 receptor subunit alpha
- Gene nameinterleukin 7 receptor
- AlternativeCD 127;CD127;CD127 antigen;CDw127;IL 7R alpha;IL 7R;IL-7 receptor subunit alpha;IL-7R subunit alpha;IL-7R-alpha;IL-7RA;IL7R;IL7RA;IL7RA_HUMAN;IL7Ralpha;ILRA;Interleukin 7 receptor alpha chain;Interleukin 7 receptor;Interleukin 7 receptor isoform H5 6;Interleukin-7 receptor subunit alpha
- Gen protein identityP16871
- Entrez nr.3575
- Gene NameIL7R
- Assay range312pg/ml-20,000pg/ml
- ImmunogenExpression system for standard: NSO; Immunogen sequence: E21-I262
- Storage & Transport ConditionsStore at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
- Cross-reactivityThere is no detectable cross-reactivity with other relevant proteins.
- Kit Components20ng/tube
- Sample Typecell culture supernates and serum.
- PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
- TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
- Gene target
- Short nameIL7R/CD127 PicoKine ELISA Kit
- TechniqueELISA kit, ELISA
- Hostantigen assay ELISA kit 1 Kit (96 Wells)
- Alternative nameH. sapiens interleukin 7 receptor/CD127 PicoKine Enzyme-linked immunosorbent assay reagent
- Alternative techniqueelisas
- Alternative to gene targetinterleukin 7 receptor, CD127 and CDW127 and IL-7R-alpha and IL7RA and ILRA, IL7R and IDBG-16161 and ENSG00000168685 and 3575, protein binding, Extracellular, Il7r and IDBG-129135 and ENSMUSG00000003882 and 16197, IL7R and IDBG-630353 and ENSBTAG00000019975 and 521554, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
EK0713 | Human IL7R/CD127 PicoKine ELISA Kitsize: 1x96 well plate | 649.95 USD
- Catalog numberEK0713
- Price649.95 USD
- Size1x96 well plate
- Gene symbolIL7R
1. Gene info
- Long gene nameinterleukin 7 receptor
- GenBank acession
- Discovery year1991-08-07
- Entrez gene record
- Pubmed identfication
- CD molecules
- Fibronectin type III domain containing
- Interleukin receptors
- Havana BLAST/BLAT
- Locus Specific Databases
- ConceptScope note:Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
- Tree numbers
- PubMedCD4(+)CD25(+)IL-7Ralpha(+) cell population represents a specific marker to monitor allospecific CD4 T cell responses in blood and in tissues after organ transplantation.IL2RA and IL7RA genes confer susceptibility for multiple sclerosis in two independent European populations.CD127 uniquely enables the purification of FOXP3+ Treg cells and, potentially, also "adaptive" regulatory T-cell subsets from the CD4+CD25- T-cell population.Expression of transgenic IL-7 receptor alpha by itself does not support increased survival of effector antigen-specific CD4 or CD8 T cells into the memory phase following infection with Listeria monocytogenes.genetic, immunologic, and metabolic factors contribute to a dysregulation of the IL-7/IL-7 receptor pathway in type 1 diabetes and identify a novel hyperglycemia-mediated interference of immune regulatory networks.
- Gene ontology - Biological process
- Gene ontology - Cellular component
- Gene ontology - Molecular function
- Entrez Gene
EK0713 | Human IL7R/CD127 PicoKine ELISA Kitsize: 96wells/kit, with removable strips. | 605.93 USD