Human IL-10 PicoKine ELISA Kit

Human IL-10 PicoKine ELISA Kit is available 2 times from Boster labs

  • ELISA's application
    For quantitative measurement of human IL-10 in cell culture supernatant, serum and plasma heparin, EDTA, citrate .
  • Gene tested
    Human IL-10
  • ELISA standard curve
    Concentration(pg/ml) + 0 + 7.8 + 15.6 + 31.3 + 62.5 + 125 + 250 + 500 O.D. + 0.023 + 0.080 + 0.126 + 0.226 + 0.424 + 0.749 + 1.388 + 2.493
  • ELISA kit detection limits
    7.8pg/ml to 500pg/ml(cellculturesupernatant)3.4pg/ml to 250pg/ml(humanserum,plasma)
  • ELISA's sensitivity
    < 0.5pg/ml
  • Target of this ELISA
    Natural and recombinant human IL-10
  • Cross reaction
    the ELISA does not cross react with any other proteins except from closely related species
  • ELISA's precision parameters
    intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);25;112;280;34;184;312 ELISA standard deviations 1.35;5.38;8.68;2.55;14.54;20.9 CV(%);5.4;4.8;3.1;7.5;7.9;6.7
  • ELISA kit description
    GENTAUR's human IL-10 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for IL-10 was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, S19-N178) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL-10 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human IL-10 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
  • Detector and capure antibodies
    a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
  • The ELISA contains
    Catalog number;Description;Quantity -;96-well plate precoated with anti- human IL-10 antibodies;1 ST0000-10;Lyophilized recombinant human IL-10 standard;10ng/tubex2 AR1107;Biotinylated anti- human IL-10 antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
  • Anti-coagulation
    heparin, EDTA or citrate
  • How the elisa works
    Sample the amount of 100ul/well of the standard solution at 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml human IL-10 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(heparin, EDTA, citrate) to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each human IL-10 standard solution and each sample is measured in duplicate.
  • Minutes of TMB incubation
    20-25
  • More gene info
    Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.
  • Antigen
    E.coli, S19-N178
  • Keep at
    Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
  • Uniprot
    Interleukin-10
  • Gene name
    interleukin 10
  • Alternative
    CSIF;Cytokine synthesis inhibitory factor;GVHDS;IL 10;IL-10;IL10;IL10_HUMAN;IL10A;Interleukin 10;Interleukin-10;Interleukin10;MGC126450;MGC126451;T cell growth inhibitory factor;TGIF
  • Gen protein identity
    P22301
  • Entrez nr.
    3586
  • Gene Name
    IL10
  • Assay range
    7.8pg/ml-500pg/ml(cellculturesupernates)3.4pg/ml-250pg/ml(humanserum,plasma)
  • Immunogen
    Expression system for standard: E.coli; Immunogen sequence: S19-N178
  • Storage & Transport Conditions
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
  • Cross-reactivity
    There is no detectable cross-reactivity with other relevant proteins.
  • Kit Components
    10ng/tube
  • Sample Type
    cell culture supernates, serum and plasma( heparin, EDTA, citrate).
  • Applications
    ELISA
  • Reactivity
    Human
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Gene target
  • Short name
    IL-10 PicoKine ELISA Kit
  • Technique
    ELISA kit, ELISA
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Species
    Human
  • Alternative name
    H. sapiens Interleukin-10 PicoKine Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, this GO :0000187 and activation of MAPK activity and biological process this GO :0001541 and ovarian follicle development and biological process this GO :0001669 and acrosomal vesicle and cellular component this GO :0002020 and protease binding and molecular function this GO :0002318 and myeloid progenitor cell differentiation and biological process this GO :0002320 and lymphoid progenitor cell differentiation and biological process this GO :0002327 and immature B cell differentiation and biological process this GO :0002371 and dendritic cell cytokine production and biological process this GO :0002551 and mast cell chemotaxis and biological process this GO :0002573 and myeloid leukocyte differentiation and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004714 and transmembrane receptor protein tyrosine kinase activity and molecular function this GO :0004716 and receptor signaling protein tyrosine kinase activity and molecular function this GO :0005020 and stem cell factor receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006687 and glycosphin this GO lipid metabolic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0007165 and signal transduction and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007283 and spermatogenesis and biological process this GO :0007286 and spermatid development and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008354 and germ cell migration and biological process this GO :0008360 and regulation of cell shape and biological process this GO :0008542 and visual learning and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0008584 and male this GO nad development and biological process this GO :0009314 and response to radiation and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0009898 and cytoplasmic side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0010628 and positive regulation of gene expression and biological process this GO :0010863 and positive regulation of phospholipase C activity and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016772 and transferase activity, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0019827 and stem cell maintenance and biological process this GO :0019955 and cytokine binding and molecular function this GO :0023014 and signal transduction by phosphorylation and biological process this GO :0030032 and lamellipodium assembly and biological process this GO :0030097 and hemopoiesis and biological process this GO :0030217 and T cell differentiation and biological process this GO :0030218 and erythrocyte differentiation and biological process this GO :0030318 and melanocyte differentiation and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0031274 and positive regulation of pseudopodium assembly and biological process this GO :0031532 and actin cytoskeleton reorganization and biological process this GO :0032762 and mast cell cytokine production and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0035162 and embryonic hemopoiesis and biological process this GO :0035234 and ectopic germ cell programmed cell death and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0035855 and megakaryocyte development and biological process this GO :0038093 and Fc receptor signaling pathway and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0038109 and Kit signaling pathway and biological process this GO :0038162 and erythropoietin-mediated signaling pathway and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042511 and positive regulation of tyrosine phosphorylation of Stat1 protein and biological process this GO :0042517 and positive regulation of tyrosine phosphorylation of Stat3 protein and biological process this GO :0042523 and positive regulation of tyrosine phosphorylation of Stat5 protein and biological process this GO :0042629 and mast cell granule and cellular component this GO :0042803 and protein homodimerization activity and molecular function this GO :0043069 and negative regulation of programmed cell death and biological process this GO :0043303 and mast cell degranulation and biological process this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0043410 and positive regulation of MAPK cascade and biological process this GO :0043473 and pigmentation and biological process this GO :0043552 and positive regulation of phosphatidylinositol 3-kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0045747 and positive regulation of Notch signaling pathway and biological process this GO :0046427 and positive regulation of JAK-STAT cascade and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048066 and developmental pigmentation and biological process this GO :0048070 and regulation of developmental pigmentation and biological process this GO :0048103 and somatic stem cell division and biological process this GO :0048170 and positive regulation of long-term neuronal synaptic plasticity and biological process this GO :0048565 and digestive tract development and biological process this GO :0048863 and stem cell differentiation and biological process this GO :0050673 and epithelial cell proliferation and biological process this GO :0050910 and detection of mechanical stimulus involved in sensory perception of sound and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0060326 and cell chemotaxis and biological process this GO :0060374 and mast cell differentiation and biological process this GO :0070662 and mast cell proliferation and biological process this GO :0097067 and cellular response to thyroid hormone stimulus and biological process this GO :0097324 and melanocyte migration and biological process this GO :0097326 and melanocyte adhesion and biological process, transferase activity, this GO :0002020: protease binding and also this GO :0004672: protein kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0004714: transmembrane receptor protein tyrosine kinase activity and also this GO :0004716: receptor signaling protein tyrosine kinase activity and also this GO :0005020: stem cell factor receptor activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, transferring phosphorus-containing groups and also this GO :0019955: cytokine binding and also this GO :0042803: protein homodimerization activity and also this GO :0046872: metal ion binding, this GO :0002020: protease binding, this GO :0004672: protein kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0004714: transmembrane receptor protein tyrosine kinase activity, this GO :0004716: receptor signaling protein tyrosine kinase activity, this GO :0005020: stem cell factor receptor activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, transferring phosphorus-containing groups, this GO :0019955: cytokine binding, this GO :0042803: protein homodimerization activity, this GO :0046872: metal ion binding, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

EK0416 | Human IL-10 PicoKine ELISA Kitsize: 1x96 well plate | 494.82 USD

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EK0416 | Human IL-10 PicoKine ELISA Kitsize: 96wells/kit, with removable strips. | 492.36 USD

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