Recombinant Human Aspartate--tRNA ligase, cytoplasmic (DARS)

Products_type

Recombinant Protein
Products_short_name

[Aspartate--tRNA ligase, cytoplasmic (DARS)]
Other_names

[aspartate--tRNA ligase, cytoplasmic isoform 2; Aspartate--tRNA ligase, cytoplasmic; aspartate--tRNA ligase, cytoplasmic; aspartyl-tRNA synthetase; Aspartyl-tRNA synthetase; AspRS; Cell proliferation-inducing gene 40 protein]
Products_gene_name

[DARS]
Other_gene_names

[DARS; DARS; HBSL; aspRS; AspRS]
Purity

>85% (SDS-PAGE)
Form

Lyophilized or liquid (Format to be determined during the manufacturing process)
Storage_stability

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

Aspartate tRNA ligase cytoplasmic DARS
Gene symbol

DARS1, DARS1-AS1
Short name

Recombinant Aspartate--tRNA ligase, cytoplasmic (DARS)
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Host

E Coli or Yeast or Baculovirus or Mammalian Cell
Species

Human, Humans
Alternative name

Rec. H. sapiens Aspartate--tRNA ligase, cytoplasmic (DARS)
Alternative technique

rec

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data