Rat CYP3A4 (Cytochrome P450, family 3, subfamily A, polypeptide 4) ELISA Kit
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Catalog numberEKE62407
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PricePlease ask
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Size96 Tests
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Estimated Turnaround Time6-11 business days
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Target NameNA
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Target s alterntive nameCYP3A4
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Species ReactivityRat
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Sample TypeSerum, Plasma, Biological Fluids
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Detection Range0.31-20 ng/mL
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Sensitivity0.19 ng/mL
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PrecisionNA
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Detection MethodColorimetric
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Assay Time4.5 hours
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ApplicationFor research use only. Not for diagnostic procedures.
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Assay TypeSandwich
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Shipping ConditionsIce packs
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Storage TemperatureShort term: 4°C; Long term: see manual.
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Precaution of UseThe Stop Solution is acidic. Do not allow to contact skin or eyes.
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Shelf lifeUse Rat CYP3A4 (Cytochrome P450, family 3, subfamily A, polypeptide 4) ELISA Kit before 6 months
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SpecificityNA
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Cross ActivityNo significant cross-reactivity or interference was observed.
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Test PrincipleThis ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CYP3A4 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CYP3A4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CYP3A4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CYP3A4. The concentration of Rat CYP3A4 in samples can be calculated by comparing the OD of the samples to the standard curve.
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StabilityNA
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Quality SystemsThe kit is manufactured at ISO 9001 certified facilities.
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Research AreaNA
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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Gene target
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Gene symbolCYP3A4, CYP3A7, CYP24A1, CYP1A1, CYP51A1, CYP2D6
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Short nameCYP3A4 (Cytochrome P450, family 3, subfamily A, polypeptide 4) ELISA Kit
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TechniqueELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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HostRat, antigen assay ELISA kit 1 Kit (96 Wells)
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SpeciesRat, Rats
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Alternative nameRat CYP3A4 (Cytochrome P450, family 3, subfamily A, multipeptide 4) Enzyme-linked immunosorbent assay reagent
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Alternative techniqueelisas, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 3 subfamily A member 4
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Synonyms gene
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Synonyms gene name
- cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4
- cytochrome P450, family 3, subfamily A, polypeptide 4
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GenBank acession
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Locus
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Discovery year1990-02-24
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Entrez gene record
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Pubmed identfication
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Classification
- Cytochrome P450 family 3
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 3 subfamily A member 7
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Synonyms gene name
- cytochrome P450, subfamily IIIA, polypeptide 7
- cytochrome P450, family 3, subfamily A, polypeptide 7
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-09-14
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Entrez gene record
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Pubmed identfication
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Classification
- Cytochrome P450 family 3
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 24 subfamily A member 1
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Synonyms gene
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Synonyms gene name
- cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)
- cytochrome P450, family 24, subfamily A, polypeptide 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-09-09
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Entrez gene record
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Classification
- Cytochrome P450 family 24
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 1 subfamily A member 1
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Synonyms gene
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Synonyms gene name
- cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1
- cytochrome P450, family 1, subfamily A, polypeptide 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cytochrome P450 family 1
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 51 subfamily A member 1
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Synonyms gene
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Synonyms gene name
- cytochrome P450, 51 (lanosterol 14-alpha-demethylase)
- cytochrome P450, family 51, subfamily A, polypeptide 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-10-26
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Entrez gene record
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Pubmed identfication
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Classification
- Cytochrome P450 family 51
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 2 subfamily D member 6
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Synonyms gene
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Synonyms gene name
- cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolizing), polypeptide 6
- cytochrome P450, family 2, subfamily D, polypeptide 7 pseudogene 2
- cytochrome P450, subfamily II (debrisoquine, sparteine, etc., -metabolising), polypeptide 7 pseudogene 2
- cytochrome P450, family 2, subfamily D, polypeptide 8 pseudogene 2
- cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolising), polypeptide 8 pseudogene 2
- cytochrome P450, family 2, subfamily D, polypeptide 6
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-04-07
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Entrez gene record
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Pubmed identfication
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Classification
- Cytochrome P450 family 2
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data