Rat CYP3A4 (Cytochrome P450, family 3, subfamily A, polypeptide 4) ELISA Kit

  • Catalog number
    EKE62407
  • Price
    Please ask
  • Size
    96 Tests
  • Estimated Turnaround Time
    6-11 business days
  • Target Name
    NA
  • Target s alterntive name
    CYP3A4
  • Species Reactivity
    Rat
  • Sample Type
    Serum, Plasma, Biological Fluids
  • Detection Range
    0.31-20 ng/mL
  • Sensitivity
    0.19 ng/mL
  • Precision
    NA
  • Detection Method
    Colorimetric
  • Assay Time
    4.5 hours
  • Application
    For research use only. Not for diagnostic procedures.
  • Assay Type
    Sandwich
  • Shipping Conditions
    Ice packs
  • Storage Temperature
    Short term: 4°C; Long term: see manual.
  • Precaution of Use
    The Stop Solution is acidic. Do not allow to contact skin or eyes.
  • Shelf life
    Use Rat CYP3A4 (Cytochrome P450, family 3, subfamily A, polypeptide 4) ELISA Kit before 6 months
  • Specificity
    NA
  • Cross Activity
    No significant cross-reactivity or interference was observed.
  • Test Principle
    This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CYP3A4 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CYP3A4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CYP3A4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CYP3A4. The concentration of Rat CYP3A4 in samples can be calculated by comparing the OD of the samples to the standard curve.
  • Stability
    NA
  • Quality Systems
    The kit is manufactured at ISO 9001 certified facilities.
  • Research Area
    NA
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • About
    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Rattus norvegicus
  • Gene target
  • Gene symbol
    CYP3A4, CYP3A7, CYP24A1, CYP1A1, CYP51A1, CYP2D6
  • Short name
    CYP3A4 (Cytochrome P450, family 3, subfamily A, polypeptide 4) ELISA Kit
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Host
    Rat, antigen assay ELISA kit 1 Kit (96 Wells)
  • Species
    Rat, Rats
  • Alternative name
    Rat CYP3A4 (Cytochrome P450, family 3, subfamily A, multipeptide 4) Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    cytochrome P450 family 24 subfamily A member 1
  • Synonyms gene
  • Synonyms gene name
    • cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)
    • cytochrome P450, family 24, subfamily A, polypeptide 1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1992-09-09
  • Entrez gene record
  • Classification
    • Cytochrome P450 family 24
  • VEGA ID
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    cytochrome P450 family 51 subfamily A member 1
  • Synonyms gene
  • Synonyms gene name
    • cytochrome P450, 51 (lanosterol 14-alpha-demethylase)
    • cytochrome P450, family 51, subfamily A, polypeptide 1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1996-10-26
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Cytochrome P450 family 51
  • VEGA ID
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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