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ELISA s application
For quantitative measurement of human CHL1 in cell culture supernatant , cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
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Gene tested
Human CHL1
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ELISA standard curve
Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.018 + 0.169 + 0.232 + 0.306 + 0.502 + 0.853 + 1.462 + 2.193
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ELISA kit detection limits
156pg/ml to 10000pg/ml
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ELISA s sensitivity
< 10pg/ml
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Target of this ELISA
Natural and recombinant human CHL1
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Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
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ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);223;1080;4453;195;957;3957 ELISA standard deviations 15.39;69.12;289.45;7.99;50.72;265.12 CV(%);6.9;6.4;6.5;4.1;5.3;6.7
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ELISA kit description
GENTAUR's human CHL1 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for CHL1 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: I25-Q1096) and your samples are measured with a biotin conjugated polyclonal from goat specific for CHL1 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human CHL1 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
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Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
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The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human CHL1 antibodies;1 ST0000-10;Lyophilized recombinant human CHL1 standard;10ng/tube×2 AR1107;Biotinylated anti- human CHL1 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
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Anti coagulation
heparin or EDTA
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How the elisa works
Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human CHL1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, cell lysate, tissue homogenates, serum or plasma (heparin, EDTA) to the ELISA plate. Keep in mind that each human CHL1 standard solution and each sample be measured in duplicate.
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Minutes of TMB incubation
20-25
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More gene info
Neural cell adhesion molecule L1-like protein also known as close homolog of L1 (CHL1) is a protein that in humans is encoded by the CHL1 gene. This protein is a member of the L1 gene family of neural cell adhesion molecules. By FISH, the CHL1 gene was mapped to chromosome 3p26.1. The protein however has dynamic localisation, meaning that it has not only multiple roles in the cell, but also various locations. This protein may also play a role in the growth of certain cancers.
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Antigen
NSO, I25-Q1096
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Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Uniprot
Neural cell adhesion molecule L1-like protein
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Gene name
cell adhesion molecule L1 like
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Alternative
CALL ; Cell adhesion molecule with homology to L1CAM (close homolog of L1) ; Close homolog of L1 ; FLJ44930 ; L1 cell adhesion molecule 2 ; L1CAM2 ; MGC132578 ; Neural cell adhesion molecule ; O00533
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Gen protein identity
O00533
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Entrez nr
10752
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbol
CHL1
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Short name
CHL1/L1CAM-2 PicoKine ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Human, Humans
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Alternative name
H. sapiens cell adhesion molecule with homology to L1CAM (close homolog on L1)/L1 cellular adhesion molecule-2 PicoKine Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
cell adhesion molecule with homology to L1CAM (close homolog of L1), CALL and L1CAM2, CHL1 and IDBG-13308 and ENSG00000134121 and 10752, protein binding, Extracellular, Chl1 and IDBG-173444 and ENSMUSG00000030077 and 12661, CHL1 and IDBG-644887 and ENSBTAG00000002471 and 504415
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