Human CellExp™CNTFR/CNTFR-alpha, human recombinant

• Catalog number
  7836-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  CNTFR, CNTF R alpha
• Alternative_names
  CNTFR, CNTF R alpha
• Description
  This receptor and its cognate ligand support the survival of neurons.
• Recombinant
  Yes
• Source
  HEK 293 cells
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 1.0 EU per ug of the rh CNTFR alpha by the LAL method.
• Activity Specifications test method
  Measured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 ug/ml (100 ul/well) can bind biotinylated human CNTF with a linear ranger of 3.2 - 100 ng/ml.
• Biological activity
  Measured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 ug/ml (100 ul/well) can bind biotinylated human CNTF with a linear ranger of 3.2 - 100 ng/ml.
• Results
  Measured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 ug/ml (100 ul/well) can bind biotinylated human CNTF with a linear ranger of 3.2 - 100 ng/ml.
• Molecular Weight
  This protein rh CNTFR alpha is fused with polyhistidine tag at the C-terminus, has a calculated MW of 36.9 kDa. The predicted N-terminus is Gln 23. DTT-reduced Protein migrates as 45-48 kDa due to glycosylation.
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
• Background Information
  Ciliary neurotrophic factor receptor subunit alpha (CNTFR or CNTFR-alpha) belongs to the type I cytokine receptor family and type 3 subfamily. CNTFR contains two fibronectin type-III domains and one Ig-like C2-type (immunoglobulin-like) domain. This receptor and its cognate ligand support the survival of neurons. This receptor is most closely related to the interleukin-6 receptor. This receptor possesses an unusual attachment to the cell membrane through a glycophosphatidylinositol linkage.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  The CellExp™CNTFR/CNTFR-alpha, recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CellExp™CNTFR/CNTFR-alpha  
• Gene symbol
  CNTFR, CNTFR-AS1
• Short name
  CellExp™CNTFR/CNTFR-alpha, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Humans
• Alternative name
  H. sapiens CellExp™ciliary neurotrophic factor receptor/ciliary neurotrophic factor receptor-a, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  ciliary neurotrophic factor receptor, CNTFR and IDBG-59992 and ENSG00000122756 and 1271, cytokine binding, Plasma membranes, Cntfr and IDBG-138110 and ENSMUSG00000028444 and 12804, BT.46623 and IDBG-635903 and ENSBTAG00000015361 and 539548

Gene info

• Identity
  HGNC:2170
• Gene
  CNTFR
• Long gene name
  ciliary neurotrophic factor receptor
• GenBank acession
  • M73238
• Locus
  9p13.3
• Discovery year
  1992-09-22
• Entrez gene record
  1271
• Pubmed identfication
  • 1648265
• Classification
  • Fibronectin type III domain containing
  • Immunoglobulin like domain containing
• VEGA ID
  OTTHUMG00000019821

Gene info

• Identity
  HGNC:48712
• Gene
  CNTFR-AS1
• Long gene name
  CNTFR antisense RNA 1
• GenBank acession
  • BC038740
  • BC042806
• Locus
  9p13.3
• Discovery year
  2013-06-07
• Entrez gene record
  415056
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000000441

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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