Human CellExp™ SCF, Human Recombinant
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Catalog number
6478-10
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Price
Please ask
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Size
10 µg
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Synonyms
Human Cellexp Human Recombinant SCF
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Alternative_names
Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kit l, DKFZp686F2250.
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Description
A cytokine playing an important role in hematopoiesis, spermatogenesis, & melanogenesis
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Recombinant
Yes
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Source
Human 293 cell expressed
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Purity by SDS PAGE
> 95%
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Assay
SDS-PAGE
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Endotoxin Level
< 1.0 EU per 1 ug of protein
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Biological activity
ED₅₀ is typically 5 to 25 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
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Results
5 to 25 ng/ml
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Molecular Weight
35 to 45 kDa, monomer, glycosylated
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Storage Temp
-20°C
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Shipping
gel pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized in PBS.
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Reconstitution Instructions
Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
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Background Information
Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms; cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
KITLG
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Short name
CellExp™ SCF, Recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ SCF, H. sapiens Rec.
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
KIT ligand
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1991-06-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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