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Synonyms
LIFR, CD118, FLJ98106, FLJ99923, LIF-R, SJS2, STWS, SWS, Leukemia inhibitory factor receptor, Cluster of Differentiation 118
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Alternative_names
LIFR, CD118, FLJ98106, FLJ99923, LIF-R, SJS2, STWS, SWS, Leukemia inhibitory factor receptor, Cluster of Differentiation 118
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Description
A polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥96%
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Assay
SDS-PAGE
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Biological activity
Measured by its ability to inhibit LIF-dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is typically 5-10 ug/ml in the presence of 0.3 ng/ml of recombinant human LIF.
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Results
Measured by its ability to inhibit LIF-dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is typically 5-10 ug/ml in the presence of 0.3 ng/ml of recombinant human LIF.
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Molecular Weight
This protein is fused with the Fc region of human IgG1 at the CT. The reduced monomer has a calculated molecular mass of 115.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhLIFR-Fc monomer is approximately 135-150 kDa due to the glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background Information
Leukemia inhibitory factor receptor also known as LIFR; CD118; FLJ98106; FLJ99923; LIF-R; SJS2; STWS; SWS, is the receptor for leukemia inhibitory factor (LIF). The leukemia inhibitory factor is a polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIF action appears to be mediated through a high-affinity receptor complex composed of a low-affinity LIF binding chain (LIF receptor) and a high-affinity converter subunit, gp130. Both LIFR and gp130 are members of a family of cytokine receptors that includes components of the receptors for the majority of hematopoietic cytokines and for cytokines that affect other systems, including the ciliary neurotrophic factor, growth hormone and prolactin Defects in LIFR are the cause of Stueve-Wiedemann syndrome (SWS), a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
LIFR, LIFR-AS1
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Short name
CellExp™ LIFR /CD118, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ leukemia inhibitory factor receptor alpha /CD118, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
leukemia inhibitory factor receptor alpha, CD118 and LIF-R and SJS2 and STWS and SWS, LIFR and IDBG-17489 and ENSG00000113594 and 3977, growth factor binding, Extracellular, Lifr and IDBG-128297 and ENSMUSG00000054263 and 16880, LIFR and IDBG-630017 and ENSBTAG00000010423 and 539504
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