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Synonyms
LDLR, FH, FHC, LDLCQ2
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Alternative_names
LDLR, FH, FHC, LDLCQ2
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Description
Mediates the endocytosis of cholesterol-rich LDL
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Recombinant
Yes
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Source
HEK 293 cells
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Purity by SDS PAGE
> 95%
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Assay
SDS-PAGE
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Endotoxin Level
< 1.0 EU per/ug
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Activity Specifications test method
Measured by its binding ability in a functional ELISA.
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Biological activity
Immobilized Human PCSK9, His Tag at 5ug/mL (100 uL/well) can bind Human LDL R, Fc Tag with a linear range of 8-320 ng/mL.
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Binding Capacity
Immobilized Human PCSK9, His Tag at 5ug/mL (100 uL/well) can bind Human LDL R, Fc Tag with a linear range of 8-320 ng/mL.
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Molecular Weight
111.4 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.
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Reconstitution Instructions
Reconstitute in sterile deionized water to the desired protein concentration.
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Background Information
Low-Density Lipoprotein (LDL) Receptor is also known as LDLR, FH, FHC, LDLCQ2, and is a mosaic protein of ~840 amino acids (after removal of signal peptide) that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). It belongs to the Low density lipoprotein receptor gene family. LDL receptor complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell. This allows LDL-cholesterol to be bound and internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface. This occurs in all nucleated cells (not erythrocytes), but mainly in the liver which removes ~70% of LDL from the circulation. Synthesis of receptors in the cell is regulated by the level of free intracellular cholesterol; if it is in excess for the needs of the cell then the transcription of the receptor gene will be inhibited. LDL receptors are translated by ribosomes on the endoplasmic reticulum and are modified by the Golgi apparatus before travelling in vesicles to the cell surface. LDL is directly involved in the development of atherosclerosis, due to accumulation of LDL-cholesterol in the blood. Atherosclerosis is the process responsible for the majority of cardiovascular diseases.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
