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Synonyms
Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein
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Alternative_names
Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein
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Description
Exerts various calcium dependent physiological activities
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Recombinant
Yes
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Source
HEK 293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Endotoxin Level
< 0.1 ng/ug of protein (
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Activity Specifications test method
Determined by its ability to inhibit Cathepsin V cleavage of a fluorogenic peptide substrate ZLR-AMC (RLUs). The expected IC50 is ≤ 100nM.
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Biological activity
Determined by its ability to inhibit Cathepsin V cleavage of a fluorogenic peptide substrate ZLR-AMC (RLUs). The expected IC50 is ≤ 100nM.
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Molecular Weight
45-55 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.8.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Background Information
Fetuin A/AHSG is a human plasma glycoprotein belonging to the Cystatin family of proteases inhibitors. It is highly expressed in embryonic cells and adult hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. Fetuin A/AHSG is a major serum protein component that exerts various calcium dependent physiological activities, and can mediate growth signaling in certain tumor cells. It also can act as a natural antagonist against specific TGF-β and BMP signaling proteins. Recombinant human Fetuin-A is a heterodimeric protein containing a 282 amino acid A chain and a 27 amino acid B chain. Due to glycosylation, recombinant Fetuin-A migrates at an apparent molecular weight of 45 – 55 kDa by SDS-PAGE run under non-reducing conditions.
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Amino acid sequence
A-Chain: APHGPGLIYR QPNCDDPETE EAALVAIDYI NQNLPWGYKH TLNQIDEVKV WPQQPSGELF EIEIDTLETT CHVLDPTPVA RCSVRQLKEH AVEGDCDFQL LKLDGKFSVV YAKCDSSPDS AEDVRKVCQD CPLLAPLNDT RVVHAAKAAL AAFNAQNNGS NFQLEEISRA QLVPLPPSTY VEFTVSGTDC VAKEATEAAK CNLLAEKQYG FCKATLSEKL GGAEVAVTCT VFQTQPVTSQ PQPEGANEAV PTPVVDPDAP PSPPLGAPGL PPAGSPPDSH VL B-Chain: TVVQPSVGAA AGPVVPPCPG RIRHFKV
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants