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Synonyms
FCGR3B, CD16B, FCG3B, FCGR3, FCG3, IGFR3
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Alternative_names
FCGR3B, CD16B, FCG3B, FCGR3, FCG3, IGFR3
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Description
Involved in activation of the NK cells for Antibody-Dependent Cell-mediated Cytotoxicity (ADCC)
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥98%
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Assay
SDS-PAGE
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Biological activity
Measured by its binding ability in a functional ELISA.
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Binding Capacity
Recombinant Human Fcγ RIIIB / CD16b can bind immobilized Anti-CD20 (Rituximab Cat. No. A1049) coated at 2 ug/ml (100 ul/well)) with a linear range of 1 - 50 ug/ml
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Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 22 kDa. The predicted N-terminus is Gly 17. DTT-reduced Protein migrates as 38-50 kDa due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered PBS (pH 7.4) with 5% trehalose.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.
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Background Information
CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner. CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16b, also known as FCGR3B and FCG3B, is expressed specifically by neutrophils and stimulated eosinophils. CD16b is the low affinity receptor for the Fc region of immunoglobulins gamma. It binds complexed or aggregated IgG and also monomeric IgG. CD16b by itself is not capable of mediating antibody-dependent cytotoxicity and phagocytosis and cannot transduce signals from outside to inside the cell but may do so by associating with cell-specific components. Soluble CD16b has a regulatory role in inflammatory processes and induces the production of pro-inflammatory cytokines.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants