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Synonyms
ERBB3, ErbB-3, HER3, HER-3, LCCS2, MDA-BF-1, MGC88033, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3, Receptor tyrosine-protein kinase erbB-3
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Alternative_names
ERBB3, ErbB-3, HER3, HER-3, LCCS2, MDA-BF-1, MGC88033, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3, Receptor tyrosine-protein kinase erbB-3
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Description
Involved in pathways leading to cell proliferation or differentiation.
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
HER3-biotin can bind immmobilized Human Her2 (ED) (Cat. No. P1160 at 5 ug/ml (100 ul/well)) with a linear range of 0.05-5 ug/ml.
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Results
HER3-biotin can bind immmobilized Human Her2 (ED) (Cat. No. P1160 at 5 ug/ml (100 ul/well)) with a linear range of 0.05-5 ug/ml.
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Molecular Weight
This protein is fused with polyhistidine tag at the C-terminus and has a calculated MW of ~70 kDa (20 – 643 aa). Under reducing conditions the protein migrates to ~93 kDa in SDS-PAGE due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered PBS (pH 7.4)
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in distilled water to a concentration up to 0.2 mg/ml.
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Background Information
ErbB3, also called human epidermal growth factor receptor 3 (HER3), is a type I membrane glycoprotein and a member of the ErbB family. ErbB family members serve as receptors for the epidermal growth factors (EGF). ErbB3 is expressed in normal adult human gastrointestinal tract, reproductive system, skin, nervous system, urinary tract, and endocrine system. ErbB3 is the only member with a defective kinase domain among ErbB family members. However, ErbB3 can form heterodimers with ErbB2 to complement a high affinity receptor complex. ErbB3 has been shown to bind the ligands heregulin and NRG-2. The lethal congenital contracture syndrome 2 (LCCS2) disease is caused by mutations affecting ErbB3. ErbB3 null mouse embryos show severely underdeveloped atrioventricular valves, leading to death at embryonic day 13.5.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
ERBB3
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Short name
CellExp™ HER3 / ErbB3 Extracellular Domain (ED), Recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ HER3 / v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) Extracellular Domain (ED), H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian), c-erbB-3 and c-erbB3 and ErbB-3 and erbB3-S and HER3 and LCCS2 and MDA-BF-1 and p180-ErbB3 and p45-sErbB3 and p85-sErbB3, ERBB3 and IDBG-39824 and ENSG00000065361 and 2065, transferase activity, Extracellular, Erbb3 and IDBG-197943 and ENSMUSG00000018166 and 13867, ERBB3 and IDBG-636302 and ENSBTAG00000010444 and 785655
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