Human CellExp™ ENPP-2 / Autotaxin, human recombinant

  • Catalog number
    7516-10
  • Price
    Please ask
  • Size
    10 ug
  • Synonyms
    ENPP2, E-NPP 2, Autotaxin, LysoPLD, ATX, PDNP2, ATX-X, NPP2, PD-IALPHA
  • Alternative_names
    ENPP2, E-NPP 2, Autotaxin, LysoPLD, ATX, PDNP2, ATX-X, NPP2, PD-IALPHA
  • Description
    ≥92% pure, active, human cell expressed, human recombinant ENPP-2 / Autotaxin - stimulates migration of melanoma cells
  • Recombinant
    Yes
  • Source
    HEK293 cells
  • Purity by SDS PAGE
    ≥92%
  • Assay
    SDS-PAGE
  • Biological activity
    Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is >8000 pmol/min/µg.
  • Results
    >8000 pmol/min/µg
  • Molecular Weight
    This protein is fused with polyhistidine tag at the N-terminus, has a calculated MW of 94.5 kDa. The predicted N-terminus is Asp 49. DTT-reduced Protein migrates as 120-130 kDa due to glycosylation.
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized
  • Physical form description
    Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
  • Reconstitution Instructions
    Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
  • Background Information
    Autotaxin also known as ectonucleotide pyrophosphatase / phosphodiesterase family member 2 (E-NPP 2 or ENPP2), Extracellular lysophospholipase D (LysoPLD), Autotaxin (ATX or ATX-X), which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP2 contains two SMB (somatomedin-B) domains. ENPP2 hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. ENPP2 can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. ENPP2 stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Gene symbol
    ENPP2, MIR7-2, MIR329-2, MIR512-2, MIR521-2, MIR1289-2, MIR509-2, KRTAP13-2, RNU6-2, SNORD116-2
  • Short name
    CellExp™ ENPP-2 / Autotaxin, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Humans
  • Alternative name
    H. sapiens CellExp™ ENPP-2 / Autotaxin, H. sapiens Rec.
  • Alternative technique
    rec
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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