Human CellExp™ Endothelial Lipase, human recombinant

Human CellExp™ Endothelial Lipase, human recombinant is available 1 time from Biovision labs

  • Target
    Endothelial Lipase
  • Background
    Lipase endothelial (LIPG) is also known as Endothelial lipase (EL/EDL). This protein has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. LIPG is designated a member of the triglyceride (TG) lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. LIPG mediates both HDL binding and uptake, and the selective uptake of HDL-CE, independently of lipolysis and CLA-1. LIPG also mediates binding and uptake of plasma lipoproteins via a process that is independent of its enzymatic activity, requires cellular heparan sulfate proteoglycans, and is regulated by ligand clustering. LIPG may have unique functional roles in the pathogenesis of coronary artery diseases such as atherosclerosis as well as in lipid metabolism in the vessel wall.
  • Concentration
    N/A
  • Molecular weight
    This protein is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 55.4 kDa. The predicted N-terminus is Ser 21. DTT-reduced Protein migrates as 55-65 kDa in SDS-PAGE due to glycosylation.
  • Synonym
    LIPG, Endothelial lipase
  • Other name
    LIPG, Endothelial lipase
  • NCBI gene number
    9388
  • NCBI gene
    LIPG
  • Gene source
    Human
  • NCBI number
    Q9Y5X9
  • Recombinant
    Yes
  • Source
    HEK 293 cells
  • Purification
    ≥92%
  • Tested applications
    SDS-PAGE, N/A
  • Level of endotoxin
    < 1.0 EU per μg of the protein by the LAL method.
  • Tested activity
    N/A
  • Biological activity
    N/A
  • Reesults
    N/A
  • Binding ability
    N/A
  • Unit
    N/A
  • Storage condition
    -20°C
  • Shipping under
    Gel Pack
  • Physical appearance
    Lyophilized
  • Physical properties
    Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
  • How to reconstitute
    Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
  • Aa sequence
    Ser 21 – Pro 500
  • Before use
    Centrifuge the vial prior to opening.
  • Notes
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Short name
    CellExp™ Endothelial Lipase, recombinant
  • Technique
    Recombinant
  • Species
    Humans
  • Alternative name
    H. sapiens CellExp™ Endothelial Lipase, H. sapiens recombinant
  • Alternative technique
    rec
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Human CellExp™ Endothelial Lipase, human recombinant
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