Human CellExp™ Cystatin C, Human Recombinant
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Catalog number
4878-50
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Price
Please ask
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Size
50 ug
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Synonyms
Human Recombinant Cystatin C
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Alternative_names
Cystatin-3, Gamma-trace neuroendocrine basic polypeptide, Post-gamma-globulin
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Description
A biomarker of kidney function
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Recombinant
Yes
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Source
Human 293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
The inhibitory function of Cystatin C on papin’s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 µg/mL (0.3 to 1.5 µM) with a range of 1.56 µg/mL to 50 µg/mL Cystatin C in presence of 0.55 µM papain and 0.44 µM L-BAPA.
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Results
Typically 0.3 to 1.5 µM IC50.
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Molecular Weight
12-13 kDa
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Storage Temp
-20°C
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Shipping
Blue ice
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Lyophilized in PBS
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Reconstitution Instructions
Reconstitute in sterile PBS.
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Background Information
Xeno-free Cystatin C HuXp (Cys-C) is expressed in human 293 cells as a monomer with an apparent molecular mass of 12 to 13 kDa. Native Cys-C in human urine is found in two different forms: one with pI 9.2 and the other with pI 7.8 by elimination of small basic peptides or amino acids from the N-terminal end of protein. Cystatin C has been studied for its role in predicting new onset or deteriorating cardiovascular disease. This cytokine is produced in a serum-free, chemically defined media.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
CellExp™ Cystatin C, Recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ Cystatin C, H. sapiens Rec.
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Alternative technique
rec
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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