Human CellExp™ Cystatin C, Human Recombinant

• Catalog number
  4878-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  Human Recombinant Cystatin C
• Alternative_names
  Cystatin-3, Gamma-trace neuroendocrine basic polypeptide, Post-gamma-globulin
• Description
  A biomarker of kidney function
• Recombinant
  Yes
• Source
  Human 293 cells
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  The inhibitory function of Cystatin C on papin’s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 µg/mL (0.3 to 1.5 µM) with a range of 1.56 µg/mL to 50 µg/mL Cystatin C in presence of 0.55 µM papain and 0.44 µM L-BAPA.
• Results
  Typically 0.3 to 1.5 µM IC50.
• Molecular Weight
  12-13 kDa
• Storage Temp
  -20°C
• Shipping
  Blue ice
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Lyophilized in PBS
• Reconstitution Instructions
  Reconstitute in sterile PBS.
• Background Information
  Xeno-free Cystatin C HuXp (Cys-C) is expressed in human 293 cells as a monomer with an apparent molecular mass of 12 to 13 kDa. Native Cys-C in human urine is found in two different forms: one with pI 9.2 and the other with pI 7.8 by elimination of small basic peptides or amino acids from the N-terminal end of protein. Cystatin C has been studied for its role in predicting new onset or deteriorating cardiovascular disease. This cytokine is produced in a serum-free, chemically defined media.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CellExp™   Cystatin  
• Short name
  CellExp™ Cystatin C, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Humans
• Alternative name
  H. sapiens CellExp™ Cystatin C, H. sapiens Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products