Human CellExp™ Carbonic Anhydrase 10/CA10, human recombinant

• Catalog number
  7485-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  CA10, Carbonic Anhydrase X, CARPX, hucep-15
• Alternative_names
  CA10, Carbonic Anhydrase X, CARPX, hucep-15
• Description
  Catalyzes the reversible hydration of carbon dioxide in various biological processes.
• Recombinant
  Yes
• Source
  HEK293 cells
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Measured by its esterase activity. The specific activity, as measured with 1 mM 4-Nitrophenyl acetate (4-NPA) as substrate and 5 ug rh CA10 / CARPX enzyme at 100 ng/µl in 100 uL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is >1 pmol/min/µg.
• Results
  Measured by its esterase activity. The specific activity, as measured with 1 mM 4-Nitrophenyl acetate (4-NPA) as substrate and 5 ug rh CA10 / CARPX enzyme at 100 ng/µl in 100 uL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is >1 pmol/min/µg.
• Molecular Weight
  This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 32.6 kDa. The predicted N-terminus is Gln 22. DTT-reduced Protein migrates as 37 kDa due to glycosylation.
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
• Background Information
  Carbonic anhydrase-related protein 10 (CA10) is also known as Carbonic anhydrase-related protein X (CARP X or CARPX), Cerebral protein 15 (hucep-15), which belongs to the alpha-carbonic anhydrase family of zinc metalloenzymes, which catalyze the reversible hydration of carbon dioxide in various biological processes. CA10 / CARPX is an acatalytic member of the alpha-carbonic anhydrase subgroup, and it is thought to play a role in the central nervous system, especially in brain development.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CellExp™   Carbonic   Anhydrase   10/CA10  
• Gene symbol
  CA10
• Short name
  CellExp™ Carbonic Anhydrase 10/CA10, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Humans
• Alternative name
  H. sapiens CellExp™ Carbonic Anhydrase 10/CA10, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:1369
• Gene
  CA10
• Long gene name
  carbonic anhydrase 10
• Synonyms gene name
  • carbonic anhydrase X
• Synonyms
  • CARPX
  • CA-RPX
  • HUCEP-15
• GenBank acession
  • AF288385
• Locus
  17q21.33-q22
• Discovery year
  1998-07-17
• Entrez gene record
  56934
• Pubmed identfication
  • 8673298
  • 9921901
• RefSeq identity
  • NM_020178
• Classification
  • Carbonic anhydrases
• VEGA ID
  OTTHUMG00000177544

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products