Human CellExp™ BTLA, Human recombinant
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Catalog number
9228-10
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Price
Please ask
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Size
10 µg
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Synonyms
BTLA, CD272
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Alternative_names
BTLA, CD272
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Description
B- and T-lymphocyte attenuator
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Recombinant
Yes
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Source
HEK 293 cells
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Purity by SDS PAGE
≥92%
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Assay
SDS-PAGE
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Purity
≥92%
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Endotoxin Level
< 1 EU/ug
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Activity Specifications test method
Measured by its binding ability in a functional ELISA
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Biological activity
Immobilized Human HVEM, C-Twin Strep Tag at 2 ng/ml (100 µl/well),can bind Human BTLA, Fc Tag with a linear of 0.1-0.5 ug/mL.
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Binding Capacity
Immobilized Human HVEM, C-Twin Strep Tag at 2 ng/ml (100 µl/well),can bind Human BTLA, Fc Tag with a linear of 0.1-0.5 ug/mL.
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Molecular Weight
This protein is fused with a human IgG1 Fc tag at C-terminus and has a calculated MW of 38.7 kDa. The protein migrates as 45-50 kDa in SDS-PAGE due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution Instructions
Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml
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Background Information
B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
BTLA
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Short name
CellExp™ BTLA, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ B and T lymphocyte associated, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
B and T lymphocyte associated, BTLA and IDBG-49858 and ENSG00000186265 and 151888, protein binding, Cell surfaces, Btla and IDBG-162900 and ENSMUSG00000052013 and 208154, BTLA and IDBG-632288 and ENSBTAG00000011871 and 531767
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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