Human CellExp Biotinylated Fc human recombinant

Human CellExp Biotinylated Fc human recombinant is available 3 times from Biovision labs

7877-5 | Human CellExp Biotinylated PD-1 / PDCD1, His Tag & Fc Tag, human recombinantsize: 5 μg | 408.35 USD

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P1004-20 | Human CellExp™ Biotinylated Fc gamma RI/CD64, human recombinantsize: 20 μg | 299.84 USD

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P1004-100 | Human CellExp™ Biotinylated Fc gamma RI/CD64, human recombinantsize: 100 μg | 1086.2 USD

  • Catalog number
    P1004-100
  • Supplier
  • Price
    1086.2 USD
  • Size
    100 μg
  • Source
    HEK 293 cells
  • Biological activity
    Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotinylated Human Fc gamma RI with a linear range of 1.5-50 ng/ml.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Short name
    CellExp™ Biotinylated Fc gamma RI/CD64, recombinant
  • Species
    Humans
  • Alternative name
    H. sapiens CellExp™ Biotinylated fragment c g RI/CD64, H. sapiens recombinant
1. Gene info
MeSH Data
  • Name
  • Concept
    Scope note:Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
  • Tree numbers
    • E01.370.225.500.363.342
    • E01.370.225.500.386.350
    • E05.196.712.516.600.240.350
    • E05.200.500.363.342
    • E05.200.500.386.350
    • E05.242.363.342
    • E05.242.386.350
Additional information
Product images
  • Target
    Fc gamma RI
  • Background
    Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses.
  • Concentration
    N/A
  • Molecular weight
    The protein has a calculated MW of 34.3 kDa. The reducing (R) protein migrates as 45-60 kDa in SDS-PAGE due to glycosylation.
  • Synonym
    High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, FcRI, Fc-gamma RIA, FcgammaRIa, CD64, FCGR1A
  • Other name
    High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, FcRI, Fc-gamma RIA, FcgammaRIa, CD64 ,FCGR1A
  • NCBI gene number
    2209
  • NCBI gene
    FCGR1A
  • Gene source
    Human
  • NCBI number
    P12314
  • Recombinant
    Yes
  • Purification
    ≥92%
  • Tested applications
    SDS-PAGE, N/A
  • Tested activity
    Fc gamma RI binding ability is measured by functional ELISA.
  • Reesults
    N/A
  • Binding ability
    Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotinylated Human Fc gamma RI with a linear range of 1.5-50 ng/ml.
  • Unit
    N/A
  • Storage condition
    -20°C
  • Shipping under
    Gel pack
  • Physical appearance
    Dry powder
  • Physical properties
    Lyophilized powder
  • How to reconstitute
    Reconstitute in sterile deionized water to a stock solution of 200 µg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% (W/V) HSA or BSA) is recommended for further dilution and long term storage.
  • Aa sequence
    Gln 16 – Pro 288
  • Before use
    Centrifuge the vial prior to opening.
  • Notes
    For Research Use Only! Not to be used in humans
  • Synonyms
    PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l
  • Alternates names
    PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l
  • Taglines
    Inhibits the T-cell proliferation and production of IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway.
  • Biomolecule/Target
    PD-1/PDCD1
  • NCBI Gene ID #.
    5133
  • NCBI Gene Symbol
    PDCD1
  • Gene Source
    Human
  • Accession #
    Q15116
  • Purity
    N/A
  • Activity (Specifications/test method)
    N/A
  • Results
    Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, Fc Tag at 5 μg/mL (100 µl/well), can bind Biotinylated Human PD-1, His Tag & Fc Tag with a linear range of 0.01-0.1 μg/mL.
  • Molecular Weight
    Biotinylated rhPDCD1 Fc Chimera, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 42.6 kDa. The predicted N-terminus is Pro 21. DTT-reduced Protein migrates as 50 kDa due to glycosylation. The primary amine of biotinylated rhPDCD1 Fc Chimera was labeled by biotin for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes.
  • Storage Temp.
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    1 year
  • Appearance
    Lyophilized
  • Physical form description
    Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
  • Reconstitution Instructions
    Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Technique
    Recombinant, FC
  • Alternative technique
    rec
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Human CellExp Biotinylated Fc human recombinant
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