Human CellExp™ Biotinylated PD-1 / PDCD1, His Tag & Fc Tag, human recombinant

  • Catalog number
    7877-5
  • Price
    Please ask
  • Size
    5 ug
  • Synonyms
    PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l
  • Alternative_names
    PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l
  • Description
    Inhibits the T-cell proliferation and production of IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway.
  • Recombinant
    Yes
  • Source
    HEK293 cells
  • Purity by SDS PAGE
    ≥95%
  • Assay
    SDS-PAGE
  • Biological activity
    Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, Fc Tag at 5 ug/mL (100 µl/well), can bind Biotinylated Human PD-1, His Tag & Fc Tag with a linear range of 0.01-0.1 ug/mL.
  • Results
    Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, Fc Tag at 5 ug/mL (100 µl/well), can bind Biotinylated Human PD-1, His Tag & Fc Tag with a linear range of 0.01-0.1 ug/mL.
  • Molecular Weight
    Biotinylated rhPDCD1 Fc Chimera, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 42.6 kDa. The predicted N-terminus is Pro 21. DTT-reduced Protein migrates as 50 kDa due to glycosylation. The primary amine of biotinylated rhPDCD1 Fc Chimera was labeled by biotin for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes.
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized
  • Physical form description
    Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
  • Reconstitution Instructions
    Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
  • Background Information
    Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. The Biotinylated Human PD-1 / PDCD1 Protein, His Tag & Fc Tag was produced using proprietary single-point enzymatic biotinlabeling technology at C-terminus to retain its native conformational structure and, therefore, bioactivity, and has been used successfully for high sensitivity and low variability assays, such as ELISA, western blotting, inhibition assay, pull-down, SPR, FACS, and affinity purification, together with anti-biotin antibody, streptavidin or avidin-conjugated probes.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Conjugation
    histidine
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    CellExp™   Biotinylated   PD-1   PDCD1   His   Tag   Tag  
  • Gene symbol
    PDCD1, ENPP2, SPATA2, SNCA, ENPP3, PDCD1LG2, CD274, IGHVII-1-1, MIR1-1, TRUND-NNN8-1
  • Short name
    CellExp™ Biotinylated PD-1 / PDCD1, His Tag & Fc Tag, recombinant
  • Technique
    Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
  • Label
    His
  • Species
    Humans
  • Alternative name
    H. sapiens CellExp™ Biotinylated PD-1 / programmed cellular death 1, histidine detection labelled & fragment c detection labelled, H. sapiens Rec.
  • Alternative technique
    rec
  • Alternative to gene target
    programmed cell death 1, CD279 and hPD-1 and hPD-l and hSLE1 and PD-1 and PD1 and SLEB2, PDCD1 and IDBG-85346 and ENSG00000188389 and 5133, protein binding, Cell surfaces, Pdcd1 and IDBG-184148 and ENSMUSG00000026285 and 18566, PDCD1 and IDBG-642702 and ENSBTAG00000011543 and 613842
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (II)-1-1 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (II)-1-1
    • immunoglobulin heavy variable (II)-1-1 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-undetermined (NNN) 8-1
  • Synonyms gene name
    • transfer RNA-undetermined (NNN) 8-1
  • GenBank acession
  • Locus
    1
  • Discovery year
    2014-06-20
  • Entrez gene record
  • Pubmed identfication
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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