Human CCKAR(Cholecystokinin A Receptor) EIA Kit
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Catalog number
E-EL-H0721-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Human
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Target Synonym
NA
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human CCKAR. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CCKAR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CCKAR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CCKAR. You can calculate the concentration of Human CCKAR in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
0.19ng/mL
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Detection Range
0.31~20ng/mL
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Target Name
CCKAR
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UNIProt ID
P32238
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Human CCKAR(Cholecystokinin A Receptor) EIA Kit
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Type
Sandwich
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Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Gene target
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Gene symbol
CCKAR
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Short name
CCKAR(Cholecystokinin A Receptor) EIA Kit
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Technique
EIA, eia
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Species
Human, Humans
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Alternative name
H. sapiens cholecystokinin A receptor(Cholecystokinin A Receptor) EIA reagent
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Alternative technique
kits, elisas
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Alternative to gene target
cholecystokinin A receptor, CCK-A and CCK1-R and CCK1R and CCKRA, CCKAR and IDBG-12723 and ENSG00000163394 and 886, cholecystokinin receptor activity, Plasma membranes, Cckar and IDBG-164710 and ENSMUSG00000029193 and 12425, CCKAR and IDBG-641476 and ENSBTAG00000004988 and 506207
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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