Recombinant Human Catechol-O-Methyltransferase

Products_type

Recombinant Protein
Products_short_name

[Catechol-O-Methyltransferase]
Products_name_syn

[COMT Human; Catechol-O-Methyltransferase Human Recombinant; COMT; EC 2.1.1.6; Catechol O-methyltransferase]
Other_names

[catechol O-methyltransferase isoform MB-COMT; Catechol O-methyltransferase; catechol O-methyltransferase; epididymis secretory sperm binding protein Li 98n; catechol-O-methyltransferase]
Products_gene_name

[COMT]
Other_gene_names

[COMT; COMT; HEL-S-98n]
Purity

Greater than 95.0% as determined by SDS-PAGE.
Form

COMT protein (1 mg/mL) in 20 mM Tris-HCI buffer, pH-8, 1 mM MgCI2 and 10% Glycerol.
Storage_stability

Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

Catechol-O-Methyltransferase
Gene symbol

CENPO, H4C15, POFUT1, SDR9C7, GNAO1, H3C15, H2BC19P, FABP3, OGT, AOPEP
Short name

Recombinant Catechol-O-Methyltransferase
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Host

Escherichia Coli.
Species

Human, Humans
Alternative name

Rec. H. sapiens Catechol-O-Methyltransferase
Alternative technique

rec

Identity

HGNC:29607
Gene

H4C15
Long gene name

H4 clustered histone 15
Synonyms gene
- HIST2H4B
Synonyms gene name
- histone 2, H4b
- histone cluster 2, H4b
- histone cluster 2 H4 family member b
Synonyms
- H4/o
GenBank acession
- BC108260
Locus

1q21.2
Discovery year

2006-06-12
Entrez gene record

554313
Pubmed identfication
- 15527963
RefSeq identity
- NM_001034077
Classification
- H4 histones
VEGA ID

OTTHUMG00000188201

Identity

HGNC:14988
Gene

POFUT1
Long gene name

protein O-fucosyltransferase 1
Synonyms
- O-FUT
- O-Fuc-T
- KIAA0180
- FUT12
Synonyms name
- peptide-O-fucosyltransferase
- GDP-fucose protein O-fucosyltransferase 1
GenBank acession
- AF375884
Locus

20q11.21
Discovery year

2001-10-29
Entrez gene record

23509
Pubmed identfication
- 9023546
- 9525914
RefSeq identity
- NM_015352
Classification
- MicroRNA protein coding host genes
- Fucosyltransferases
VEGA ID

OTTHUMG00000133268

Identity

HGNC:4389
Gene

GNAO1
Long gene name

G protein subunit alpha o1
Synonyms gene name
- guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
Synonyms
- G-ALPHA-o
Locus

16q13
Discovery year

1988-04-24
Entrez gene record

2775
Pubmed identfication
- 1899283
- 11395521
RefSeq identity
- NM_020988
Classification
- G protein subunits alpha, group i
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000133241

Identity

HGNC:20505
Gene

H3C15
Long gene name

H3 clustered histone 15
Synonyms gene
- HIST2H3A
Synonyms gene name
- histone 2, H3a
- histone cluster 2, H3a
- histone cluster 2 H3 family member a
Synonyms
- H3/n
- H3/o
GenBank acession
- AF531305
Locus

1q21.2
Discovery year

2003-02-19
Entrez gene record

333932
Pubmed identfication
- 12408966
- 15527963
Classification
- H3 histones
VEGA ID

OTTHUMG00000012094

Identity

HGNC:20517
Gene

H2BC19P
Long gene name

H2B clustered histone 19, pseudogene
Synonyms gene
- H2BFO
- HIST2H2BD
Synonyms gene name
- histone 2, H2bd
- H2B histone family, member O
- histone cluster 2, H2bd (pseudogene)
- histone cluster 2 H2B family member d (pseudogene)
Synonyms
- H2B/s
- H2B/o
GenBank acession
- AY131978
Locus

1q21.2
Discovery year

2003-02-20
Entrez gene record

337874
Pubmed identfication
- 12408966
- 15527963
RefSeq identity
- NG_001226
Classification
- H2B histones
VEGA ID

OTTHUMG00000035827

Identity

HGNC:3557
Gene

FABP3
Long gene name

fatty acid binding protein 3
Synonyms gene
- MDGI
- FABP11
Synonyms gene name
- fatty acid binding protein 11
- fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)
- fatty acid binding protein 3, muscle and heart
Synonyms
- H-FABP
- O-FABP
Synonyms name
- mammary-derived growth inhibitor
GenBank acession
- U57623
Locus

1p35.2
Discovery year

1991-08-06
Entrez gene record

2170
Pubmed identfication
- 8661024
RefSeq identity
- NM_004102
Classification
- Fatty acid binding protein family
VEGA ID

OTTHUMG00000003797

Identity

HGNC:8127
Gene

OGT
Long gene name

O-linked N-acetylglucosamine (GlcNAc) transferase
Synonyms gene name
- O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Synonyms
- O-GLCNAC
- HRNT1
- MGC22921
- FLJ23071
- OGT1
Synonyms name
- UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase
GenBank acession
- U77413
Locus

Xq13.1
Discovery year

1998-12-03
Entrez gene record

8473
Pubmed identfication
- 9083068
RefSeq identity
- NM_003605
- NM_181672
Classification
- O-linked N-acetylglucosaminyltransferases
- NSL histone acetyltransferase complex
- Tetratricopeptide repeat domain containing
VEGA ID

OTTHUMG00000033316
Locus Specific Databases
- Global Variome shared LOVD

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data