Human CASP3(Caspase 3) EIA Kit

  • Catalog number
    E-EL-H0017-96T
  • Price
    Please ask
  • Size
    96Test
  • Detection Type
    Colormetric
  • Target Species
    Human
  • Target Synonym
    CPP32, CPP32B, SCA-1, Apoptain, Yama
  • Test principle
    This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human CASP3. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CASP3. You can calculate the concentration of Human CASP3 in the samples by comparing the OD of the samples to the standard curve.
  • Sensitivity
    0.19ng/mL
  • Detection Range
    0.31~20ng/mL
  • Target Name
    CASP3
  • UNIProt ID
    P42574
  • Tested Sample Types
    Serum, Plasma, Cell supernatant
  • Product Name
    Human CASP3(Caspase 3) EIA Kit
  • Type
    Sandwich
  • Description
    Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways. This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
  • Properties
    MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Gene target
    CASP3   Caspase   Kit  
  • Gene symbol
    CASP3
  • Short name
    CASP3(Caspase 3) EIA Kit
  • Technique
    EIA, eia
  • Species
    Human, Humans
  • Alternative name
    H. sapiens caspase 3, apoptosis-related cysteine peptidase(caspase 3) EIA reagent
  • Alternative technique
    kits, elisas
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
  • Identity
  • Gene
  • Long gene name
    caspase 3
  • Synonyms gene name
    • caspase 3, apoptosis-related cysteine protease
    • caspase 3, apoptosis-related cysteine peptidase
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1996-07-22
  • Entrez gene record
    836
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Caspases
    • Small nucleolar RNA protein coding host genes
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
  • Tree numbers
    • E05.599.495
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