Human BMP-4 PicoKine ELISA Kit

  • Catalog number
    EK0314
  • Price
    Please ask
  • Size
    1x96 well plate
  • ELISA s application
    For quantitative measurement of human BMP-4 in bone tissue and cell culture supernatant.
  • Gene tested
    Human BMP-4
  • ELISA standard curve
    Concentration(pg/ml) + 0 + 62.5 + 125 + 250 + 500 + 1000 + 2000 + 4000 O.D. + 0.037 + 0.083 + 0.143 + 0.285 + 0.523 + 1.037 + 1.678 + 2.412
  • ELISA kit detection limits
    62.5pg/ml to 4000pg/ml
  • ELISA s sensitivity
    < 4pg/ml
  • Target of this ELISA
    Natural and recombinant human BMP-4
  • Cross reaction
    the ELISA does not cross react with any other proteins except from closely related species
  • ELISA s precision parameters
    intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);376;987;2136;453;1209;2351 ELISA standard deviations 15.79;45.40;117.48;23.10;68.91;141.06 CV(%);4.2;4.6;5.5;5.1;5.7;6.0
  • ELISA kit description
    GENTAUR's human BMP-4 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for BMP-4 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, S293-R408) and your samples are measured with a biotin conjugated polyclonal from goat specific for BMP-4 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human BMP-4 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
  • Detector and capure antibodies
    a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
  • The ELISA contains
    Catalog number;Description;Quantity -;96-well plate precoated with anti- human BMP-4 antibodies;1 ST0000-10;Lyophilized recombinant human BMP-4 standard;10ng/tube×2 AR1107;Biotinylated anti- human BMP-4 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
  • Anti coagulation
    /
  • How the elisa works
    Sample the amount of 100ul/well of the standard solution at 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human BMP-4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human bone tissue or cell culture supernatant to the ELISA plate. Keep in mind that each human BMP-4 standard solution and each sample be measured in duplicate.
  • Minutes of TMB incubation
    15-20
  • More gene info
    Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23.1, The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog(released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal(by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.
  • Antigen
    NSO, S293-R408
  • Keep at
    Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
  • Uniprot
    Bone morphogenetic protein 4
  • Gene name
    bone morphogenetic protein 4
  • Alternative
    BMP 2B;BMP 4;BMP2B;BMP2B1;BOMPR4A;Bone morphogenetic protein 2B;Bone morphogenetic protein 4;DVR4; OTTMUSP00000021065;OTTMUSP00000021066;ZYME
  • Gen protein identity
    P12644
  • Entrez nr
    652
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Gene target
    BMP-4   PicoKine  
  • Gene symbol
    BMP4
  • Short name
    BMP-4 PicoKine ELISA Kit
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Species
    Human, Humans
  • Alternative name
    H. sapiens BMP-4 PicoKine Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
Product images
Similar products
Filters
Contact
Chat with gentaur.com employee