Human anti-cardiolipin antibody(IgM) ELISA Kit
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Long name
ELISA test kit for Human anti-cardiolipin antibody(IgM)
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Synonym name
Homo sapiens anti-cardiolipin antibody(IgM) ELISA Kit
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General description
This is a ready to use kit for performing ELISA tests for detection of anti-cardiolipin antibody(IgM) in samples from Human
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Antigen
anti-cardiolipin antibody(IgM)
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Species reactivity
Human
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Sample types
Our anti-cardiolipin antibody(IgM) ELISA kit has been proven to work and successfully detect anti-cardiolipin antibody(IgM) in Human
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Kit contents
ELISA test kit for Human anti-cardiolipin antibody(IgM)Assay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual
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Detection wavelengt
450 nm
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Assay procedure
1-4h
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Summary of the prodecure
1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.
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Applications
Enzyme Linked Immunosorbent Assay
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Shipping storage shelf life
The anti-cardiolipin antibody(IgM) ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Human anti-cardiolipin antibody(IgM) ELISA Kit is 6 months after delivery.
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Tips
Due to transportation and handling small amounts of the liquid components of the Homo sapiens anti-cardiolipin antibody(IgM) ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by SAB ELISA KIT they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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French translation
anticorps
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Gene target
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Gene symbol
CRLS1, PKN2
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Short name
anti-cardiolipin antibody(IgM) ELISA
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Technique
ELISA kit, Antibody, anti-, ELISA, anti, IgM, antibody to, antibodies, antibodies against human proteins, antibodies for, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. IgMs
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Isotype
IgM, IgM
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Species
Human, Humans
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Alternative name
H. sapiens Antibody tocardiolipin (antibody to-)(Immunoglobulin M) Enzyme-linked immunosorbent assay reagent
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Alternative technique
antibodies, elisas, kits, immunoglobulin-m
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
cardiolipin synthase 1
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Synonyms gene
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Synonyms gene name
- chromosome 20 open reading frame 155
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2001-07-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
protein kinase N2
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Synonyms gene
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Synonyms gene name
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1997-10-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C2 domain containing protein kinases
- AGC family kinases
- Small nucleolar RNA protein coding host genes
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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