Human A-GHRL (Acylated Ghrelin) ELISA Kit
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Catalog numberEKE61138
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PricePlease ask
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Size96 Tests
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Estimated Turnaround Time6-11 business days
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Target NameNA
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Target s alterntive nameA-GHRL
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Species ReactivityHuman
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Sample TypeSerum, Plasma, Biological Fluids
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Detection Range15.63-1000 pg/mL
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Sensitivity9.38 pg/mL
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PrecisionNA
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Detection MethodColorimetric
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Assay Time4.5 hours
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ApplicationFor research use only. Not for diagnostic procedures.
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Assay TypeSandwich
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Shipping ConditionsIce packs
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Storage TemperatureShort term: 4°C; Long term: see manual.
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Precaution of UseThe Stop Solution is acidic. Do not allow to contact skin or eyes.
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Shelf lifeUse Human A-GHRL (Acylated Ghrelin) ELISA Kit before 6 months
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SpecificityNA
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Cross ActivityNo significant cross-reactivity or interference was observed.
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Test PrincipleThis ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human A-GHRL . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human A-GHRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human A-GHRL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human A-GHRL. The concentration of Human A-GHRL in samples can be calculated by comparing the OD of the samples to the standard curve.
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StabilityNA
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Quality SystemsThe kit is manufactured at ISO 9001 certified facilities.
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Research AreaNA
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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DescriptionGhrelin, GHRL gene, obestatin prepropeptide or lenomorelin from ghrelinergic cells in the gastrointestinal tract. GHRL is a neuropeptide in the central nervous system regulating appetite. Ghrelin is regulating the distribution and rate of use of body energy.
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Gene target
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Gene symbolGHRL, GHRLOS, HLA-A, IGHV5-10-1, CIDEA, RPA1, LINC00852, CDC37P1, OR1Q1
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Short nameA-GHRL (Acylated Ghrelin) ELISA Kit
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TechniqueELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Hostantigen assay ELISA kit 1 Kit (96 Wells)
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SpeciesHuman, Humans
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Alternative nameH. sapiens A-ghrelin/obestatin prepropeptide (Acylated Ghrelin) Enzyme-linked immunosorbent assay reagent
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Alternative techniqueelisas, kits
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Alternative to gene targetghrelin/obestatin prepropeptide, GHRL and IDBG-18011 and ENSG00000157017 and 51738, ghrelin receptor binding, Extracellular, Ghrl and IDBG-178411 and ENSMUSG00000064177 and 58991, GHRL and IDBG-646311 and ENSBTAG00000012328 and 281192
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Gene info
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Identity
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Gene
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Long gene nameghrelin and obestatin prepropeptide
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Synonyms gene name
- ghrelin, growth hormone secretagogue receptor ligand
- ghrelin/obestatin prepropeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-01-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Neuropeptides
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameghrelin opposite strand/antisense RNA
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Synonyms gene name
- ghrelin opposite strand RNA (non-protein coding)
- ghrelin opposite strand/antisense RNA (non-protein coding)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2007-11-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long non-coding RNAs with non-systematic symbols
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, A
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 5-10-1
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
- immunoglobulin heavy variable 5-A (provisional)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecell death inducing DFFA like effector a
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Synonyms gene name
- cell death-inducing DFFA-like effector a
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namereplication protein A1
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Synonyms gene name
- replication protein A1 (70kD)
- replication protein A1, 70kDa
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Nucleotide excision repair
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelong intergenic non-protein coding RNA 852
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Synonyms gene
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Synonyms gene name
- chromosome 3 open reading frame 42
- ghrelin opposite strand/antisense RNA 2 (tail to tail)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-01-10
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Entrez gene record
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Pubmed identfication
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Classification
- Long intergenic non-protein coding RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecell division cycle 37 pseudogene 1
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Synonyms gene name
- cell division cycle 37 homolog (S. cerevisiae) pseudogene 1
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Synonyms
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Locus
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Discovery year2009-01-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 1 subfamily Q member 1
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Synonyms gene
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Synonyms gene name
- olfactory receptor, family 1, subfamily Q, member 1
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Synonyms
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Locus
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Discovery year1999-12-09
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Entrez gene record
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Classification
- Olfactory receptors, family 1
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VEGA ID
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data