HSC70 Antibody / HSPA8

• Catalog number
  R31940
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  HSC70 / HSPA8
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.1-0.5ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  Optimal dilution of the HSC70 antibody should be determined by the researcher.
• Intented use
  This HSC70 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P11142
• Purity
  Antigen affinity
• Description
  HSPA8 (heat shock 70kDa protein 8) also known as HSC70, HSC71, HSP73, HSPA10, FORMERLY, LAP1 or LPS-ASSOCIATED PROTEIN 1, is a heat shock protein that in humans is encoded by the HSPA8 gene. The HSPA8 gene contains 9 exons and spans 5 kb. The deduced HSPA8 protein has 646 amino acids and a predicted molecular mass of 70,899 Da. And the HSPA8 gene is mapped on 11q24.1. HSPA8 plays an important role in cells by transiently associating with nascent polypeptides to facilitate correct folding. HSP73 also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Rapid decay involves AU-rich binding protein AUF1, which complexes with heat-shock proteins HSC70 and HSP70, translation initiation factor EIF4G, and poly (A)-binding protein. In the absence of Il3, Hsc70 formed a complex with Hsp40 and Hip, and this complex, in association with Eif4g and Pabp, formed a high-stability complex with Bim mRNA that protected it from ribonucleases.
• Immunogen
  Amino acids 520-614 of human HSPA8/HSC70 were used as the immunogen for the HSC70 antibody.
• Storage
  After reconstitution, the HSC70 antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  HSC70   HSPA8  
• Gene symbol
  HSPA8
• Short name
  Anti-HSC70 / HSPA8
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to HSC70 / HSPA8
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:5241
• Gene
  HSPA8
• Long gene name
  heat shock protein family A (Hsp70) member 8
• Synonyms gene
  • HSPA10
• Synonyms gene name
  • heat shock 70kD protein 8
  • heat shock 70kDa protein 8
• Synonyms
  • HSC71
  • HSC70
  • HSP73
• GenBank acession
  • Y00371
• Locus
  11q24.1
• Discovery year
  1995-05-08
• Entrez gene record
  3312
• Pubmed identfication
  • 8530083
  • 3037489
• Classification
  • Spliceosomal P complex
  • Heat shock 70kDa proteins
  • Spliceosomal C complex
• VEGA ID
  OTTHUMG00000166030

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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