Hoyle Medium Base

Description

Hoyle Medium Base is a selective mediium, reported to isolate and differentiate Corynebacterium diphtheriae types.
Other Product
Alternative Name

Hoyle Medium Base for Corynebacterium diphtheriae
Type

Dehydrated Culture Media
Origin

Animal
Classification

Bacterial
Composition g L

Peptone 10.000 Meat extract B 10.000 Sodium chloride 5.000 Agar 15.000 Final pH ( at 25°C) 7.8±0.2
Quantity required per litre

40 g
Appearance

Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium

Basal Medium: Amber coloured, clear to slightly opalescent gel After Addition of blood & Tellurite : Brownish red coloured opaque gel forms in Petri plates
Cultural response

Cultural characteristics observed with added 50 ml of laked blood and tellurite solution, after an incubation at 35-37°C for 18-24 hours .
Storage

Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C.
Shelf Life

Use before expiry date on the label.
Technical Data
Data Sheet
Additional description

There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
Group

media

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data