Horse Anti-Mouse/Rabbit Polyclonal IgG, biotinylated

  • Catalog number
    ZU114
  • Price
    Please ask
  • Size
    1 ml
  • Availability
    Please contact us to check the availability
  • Immunogen
    Affinity purified IgG from mouse and rabbit
  • Specificity
    cross-reactivities are batch dependent, please enquire!
  • Species reactivity
    Mouse, rabbit (rat)
  • Product presentation
    Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
  • Product category
    Secondary Antibodies
  • Antibody s raised in
    Horse
  • Antibody s isotype
    IgG
  • Antibody s clone
    polyclonal
  • Product is supplied as
    liquid concentrate
  • Recommended concentration for use
    dilute 1:50 up to 1:250
  • Protein concentration
    700 µg/ml
  • Positive control
    none
  • Pretreatment
    none
  • Tested applications
    IHC(C, P), ELISA, WB
  • Recommended secondary reagent
    We recommend the use of Gentaur's Streptavidin-HRPO (Art. No. ZU054) and Streptavidin-AP (Art. No. ZU051).
  • Storage method
    Store at 2-8°C
  • UniProt number
    Go to UniProt/SwissProt
  • Litterature
    Go to PubMed
  • Tips
    Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Oryctolagus cuniculus
  • Group
    Polyclonals and antibodies
  • Gene target
  • Short name
    Secondary Reagents
  • Technique
    Polyclonal, Rabbit, anti-, Mouse, anti, IgG, antibody to, antibodies, IgGs, mouses, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Horse, Rabbits
  • Isotype
    IgG, IgG
  • Species
    Mouse, Horses, Mouses
  • Alternative name
    Anti-Mouse/Rabbit IgG, biotinylated
  • Alternative technique
    polyclonals, rabbit-anti, murine, antibodies, immunoglobulins
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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