HMG-CoA Reductase Catalytic Domain (GST Tagged), human recombinant

• Catalog number
  7841-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  3-Hydroxy-3-methylglutaryl-CoA reductase, HMGR, HMGCR
• Alternative_names
  3-Hydroxy-3-methylglutaryl-CoA reductase, HMGR, HMGCR
• Description
  ≥90% Pure Human recombinant GST Tagged HMG-CoA Reductase Catalytic Domain, a primary target of statins.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  The specific activity was determined to be > 1.0 Unit/mg by BioVision’s HMG-CoA reductase Activity/Inhibitor Screening Kit (Cat # K588).
• Results
  > 1.0 Unit/mg
• Unit Definition
  One unit is defined as the amount of enzyme required to convert 1.0 umole of NADPH to NADP+ per minute at 37 °C
• Molecular Weight
  75.9 kDa (426–888 aa, NT GST Tag)
• Storage Temp
  -80°C
• Shipping
  dry ice
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from proprietary buffer containing BSA
• Reconstitution Instructions
  Briefly spin down the vial and reconstitute in 50% glycerol in PBS to 0.5-1.0 mg/ml and store at –80°C.
• Background Information
  HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGR) (EC 1.1.1.34) is a transmembrane protein involved in the mevalonate pathway, the metabolic pathway that produces cholesterol from acetyl-CoA. In an NADPH-dependent reaction, HMG-CoA reductase reduces HMG-CoA to generate mevalonate and CoA. Since HMG-CoA reductase catalyzes the rate limiting step in cholesterol synthesis, it is a primary target of a group of cholesterol-lowering drugs known as statins. Inhibition of HMG-CoA reductase induces expression of LDL receptors in the liver, which lowers plasma concentration of cholesterol.
• Amino acid sequence
  426–888 aa
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  HMG-CoA   Reductase   Catalytic   Domain   GST   Tagged  
• Gene symbol
  MGST3
• Short name
  HMG-CoA Reductase Catalytic Domain (GST Tagged), recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  HMG-CoA Reductase Catalytic Domain (GST Tagged), H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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