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Description
HM Peptone B, Certified is a culture media product of animal origin (beef), it's a combination of different nutrient for developping a large part of microoraganisms in culture media, used also in the production of antibiotics, enzymes and other product
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Appearance
Light yellow to brownish yellow homogenous free flowing powder, having characteristic odour but not putrescent
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Solubility
Freely soluble in distilled/purified water, insoluble in alcohol.
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Clarity
1% w/v aqueous solution remains clear without haziness after autoclaving at 15 lbs pressure (121ºC) for 15 minutes.
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Reaction
Reaction of 2% w/v aqueous solution at 25ºC.
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PH
5.90- 6.90
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Total aerobic microbial count cfu gm
By plate method when incubated at 30-35°C for not less than 3 days. Bacterial Count :
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Total Yeast and mould count cfu gm
By plate method when incubated at 20-25°C for not less than 5 days. Yeast & mould Count :
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Test for Pathogens
1. E.coli-Negative in 10 gms of sample2. Salmonella species-Negative in 10 gms of sample3. Pseudomonas aeruginosaNegative in 10 gms of sample4. Staphylococcus aureus- Negative in 10 gms of sample5. C.albicans- Negative in 10 gms of sample6. Clostridia- Negative in 10 gms of sample
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Indole Test
Tryptophan content: Passes
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Cultural response
Cultural response observed after an incubation at 35-37ºC for 18-24 hours by preparing Nutrient Agar (M001) using HM Peptone B, certified as an ingredient.
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Chemical Analysis
TotalNitrogen >= 12.0% AminoNitrogen >= 3.80% Sodium chloride
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Storage
Store between 10-30°C in tightly closed container and away from bright light.
