HIV1 Nef ELI protein FITC

• Catalog number
  65-025003F
• Price
  Please ask
• Size
  100 µg

• Category
  Proteins
• Antibody Subtype
  Conjugated Proteins
• Area of research
  Infectious Disease
• Reactivity
  Viral
• Conjugate Tag
  FITC
• Type of protein
  Recombinant
• Expression System
  Escherichia coli
• Grade Purity
  Grater than 90% pure
• Concentration
  1.0 mg/ml (lot specific)
• Form Buffer
  Provided as a liquid in PBS buffer.
• Storage
  Store frozen product at -70 deg C or lower. Avoid repeated freeze/thaw cycles
• Shipping conditions
  Dry Ice
• Tested for
  ELISA; WB
• Usage Recommendations
  ELISA: 1:500-1:1000, WB: 1:500-1:1000
• URL
• More info
• Properties
  This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  HIV1   Nef   ELI   protein  
• Short name
  HIV1 Nef ELI protein FITC
• Technique
  FITC, Fluorescein
• Label
  FITC
• Alternative name
  Purified recombinant HIV1 Nef ELI (FITC)
• Alternative technique
  fluorescine
• Virus
  hiv1

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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