HIV Reverse Transcriptase Assay Kit (enzyme not included)
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Catalog number
HIV100K -100
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Price
Please ask
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Size
100 assays
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Full description
HIV Reverse Transcriptase Assay Kit (enzyme not included) includes 400 µl of 10 x Buffer, 33 µl of 100 x Template, 33 µl of 100 x dNTPs, 1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA. It is for 100 assays of HIV Reverse Transcriptase. The assay kit includes all reagents except the enzyme.
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Partial description
Reagents for fluorescence-based 384-well l assays of HIV Reverse Transcriptase activity, 100 assays, enzyme is not included
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Product type
HIV Reverse Transcriptase Assay
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Shipped to you
This product is shipped to you frozen
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Ordering
Product made-to-order. Delivery is 1-2 weeks upon order confirmation. Please contact us to check the current inventory stock. For larger or bulk quantity a discount may apply.
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Precautions
For Research Use Only. Produced in USA
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Test
ProF supplies other types of Assays as 1.
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Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional. Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
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Gene
The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome(AIDS). AIDS is a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive. Without treatment, average survival time after infection with HIV is estimated to be 9 to 11 years, depending on the HIV subtype. Infection with HIV occurs by the transfer of blood, semen, vaginal fluid, pre-ejaculate, or breast milk. Within these bodily fluids, HIV is present as both free virus particles and virus within infected immune cells. recombinant HIV 1 and 2 gag gene proteins p24, p17, p55 immunodominant epitopes and envelope glycoproteins, gp120 are used for production of diagnostic detection antibodies.
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Goup
reverse transcription
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Gene target
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Gene symbol
HIVEP2
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Short name
HIV Reverse Transcriptase Assay Kit (enzyme not included)
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Technique
reverse, Enzyme, Assay, assays, enzymes
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Host
Assay
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Alternative name
human immunodeficiency virus Reverse Transcriptase test reagent (enzyme not included)
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Alternative technique
transctription, enzymes, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Virus
hiv
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Gene info
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Identity
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Gene
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Long gene name
HIVEP zinc finger 2
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Synonyms gene name
- human immunodeficiency virus type I enhancer-binding protein 2
- human immunodeficiency virus type I enhancer binding protein 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1992-10-21
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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