HIV-1 Reverse transcriptase, active, SDS-PAGE, WB, Dot, ELISA
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Catalog number
05-002
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Price
Please ask
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Size
1000units
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Test
Dot blots are simpler than Western blot since no electrophoresis needed. It is advised to use PVDF or Nitrocellulose to deposit the protein samples that will be incubated with the blotting serum before ECL or colorimetric detection. ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Gene
The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome(AIDS). AIDS is a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive. Without treatment, average survival time after infection with HIV is estimated to be 9 to 11 years, depending on the HIV subtype. Infection with HIV occurs by the transfer of blood, semen, vaginal fluid, pre-ejaculate, or breast milk. Within these bodily fluids, HIV is present as both free virus particles and virus within infected immune cells. recombinant HIV 1 and 2 gag gene proteins p24, p17, p55 immunodominant epitopes and envelope glycoproteins, gp120 are used for production of diagnostic detection antibodies.
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Description
Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
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Goup
reverse transcription
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Gene target
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Gene symbol
SDS, PAGE1, SDSL, PAGE5, PAGE4, PAGE3, PAGE2, SBDS, HIVEP2, IGHVII-1-1
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Short name
HIV-1 Reverse transcriptase, active, SDS-PAGE, , Dot, ELISA
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Technique
reverse, ELISA, page, dot, Dot, sds, WB, cicular dots, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. Western Blot on a membrane
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Alternative name
human immunodeficiency virus-1 Reverse transcriptase, functionnal, SDS-PAGE, Western Blot, Dot, Enzyme-linked immunosorbent assay
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Alternative technique
transctription, elisas, blots
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Virus
hiv
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Gene info
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Identity
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Gene
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Long gene name
serine dehydratase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1999-08-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
PAGE family member 1
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Synonyms gene
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Synonyms gene name
- G antigen, family B, 1 (prostate associated)
- P antigen family, member 1 (prostate associated)
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Synonyms
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GenBank acession
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Locus
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Discovery year
1999-04-15
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene name
serine dehydratase like
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Synonyms gene name
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Synonyms
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GenBank acession
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Locus
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Discovery year
2004-02-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
PAGE family member 5
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Synonyms gene name
- P antigen family, member 5 (prostate associated)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2005-03-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
PAGE family member 4
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Synonyms gene
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Synonyms gene name
- G antigen, family C, 1
- P antigen family, member 4 (prostate associated)
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Synonyms
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GenBank acession
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Locus
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Discovery year
1999-04-15
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
PAGE family member 3
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Synonyms gene
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Synonyms gene name
- G antigen, family D, 1
- P antigen family, member 3 (prostate associated)
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Synonyms
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Locus
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Discovery year
1999-04-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene name
PAGE family member 2
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Synonyms gene
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Synonyms gene name
- G antigen, family C, 2
- P antigen family, member 2 (prostate associated)
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Synonyms
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GenBank acession
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Locus
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Discovery year
2005-03-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
Gene info
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Identity
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Gene
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Long gene name
HIVEP zinc finger 2
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Synonyms gene name
- human immunodeficiency virus type I enhancer-binding protein 2
- human immunodeficiency virus type I enhancer binding protein 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1992-10-21
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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