Histone H3, monomethyl (Lys4)

Products_type

Antibody
Products_short_name

[Histone H3, monomethyl]
Products_name_syn

[Anti -Histone H3, monomethyl (Lys4)]
Other_names

[histone H3; histone H3]
Other_gene_names

[H3]
Clonality

Polyclonal
Reactivity

Human
Specificity

Recognizes human monomethyl-histone H3 (Lys4), Mr 17kD. Specificity was confirmed by the ability of 1uM of the immunizing peptide to completely abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts. No competition was observed with peptides containing dimethyl-lysine 4, trimethyl-lysine 4, mono-methyllysine 4 or monomethyl-lysine 9 or 27.
Purity

Affinity Purified Purified by Protein A affinity chromatography.
Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Storage_stability

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Tested_application

Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)