Histone H3 Antibody (tri Methyl Lys4): FITC
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Catalog number
SMC-252D-FITC
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Price
Please ask
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Size
100 µl
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Alternative Name
Histone H3 Antibody (tri Methyl Lys4), Clone 2E11: FITC
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Other name
Mouse Anti-Human Histone H3 (tri Methyl Lys4) Monoclonal IgG1
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Target
Histone H3
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Conjugate
FITC
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Category
Antibodies
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Product Type
Monoclonal
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Clone Number
2E11
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Immunogen
Prepared by immunizing synthetic peptide coupled to KLH.
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Immunogen Species
Human
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Gene ID
NA
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Swiss Prot
P68431/Q71DI3/P84243
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Applications
WB
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Host Species
Mouse
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Species Reactivity
Human , Mouse , Rat
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Storage Buffer
PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
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Concentration
NA
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Specificity
Detects endogenous Histone H3 (tri methyl K4) protein.
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Storage Temperature
-20°C
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Shipping Temperature
Blue Ice or 4°C
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Properties
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Description
A methyl group is an alkyl derived from methane, containing one carbon atom bonded to three hydrogen atoms — CH3. In formulas, the group is often abbreviated Me. Such hydrocarbon groups occur in many organic compounds. It is a very stable group in most molecules. While the methyl group is usually part of a larger molecule, it can be found on its own in any of three forms anion, cation or radical. The anion has eight valence electrons, the radical seven and the cation six. All three forms are highly reactive and rarely observed,
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French translation
anticorps
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Gene target
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Short name
Histone H3 Antibody (tri Methyl Lys4): FITC
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Isotype
IgG1
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Label
FITC
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Alternative name
Histone H3 (antibody to-) (tri Methyl Lys4): fluorecein
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Alternative technique
antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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