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Category
Proteins & Peptides
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Long description
This peptide is the immunogen used to make Cat # X2752P. _x000B__x000B_Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
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Antibody come from
Synthetic peptide derived from human Chromogranin A protein
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
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Clone
not specified
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Antigen antibody binding interaction
Histone H2a.x (pS140) - Blocking Peptide
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Antibody is raised in
see techfile
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Antibody s reacts with
Blocking Peptide to Cat # X2752P
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Blocking Peptide
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Antibody s suited for
Antibody can be used for immunohistochemistry (1-3 µg/ml) and Western blotting (1-2 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Konecki, D.S., et al. ÔThe primary structure of human chromogranin A and pancreastatinÕ J. Biol. Chem., 262, 17026-17030 (1987)_x000B_2. Pasqua, T., et al. ÔFull-length human chromogranin-A cardioactivity: myocardial, coronary, and stimulus-induced processing evidence in normotensive and hypertensive male rat hearts.Õ Endocrinology, 154, 3353-3365 (2013)_x000B_3. Chen, Y., et al. ÔNaturally occurring genetic variants in human chromogranin A (CHGA) associated with hypertension as well as hypertensive renal disease.Õ Cell Mol. Neurobiol., 30, 1395-1400 (2010)_x000B_4. Mahapatra, N.R., et al. ÔHypertension from targeted ablation of chromogranin A can be rescued by the human ortholog.Õ J. Clin. Invest., 115, 1942-1952 (2005)_x000B_5. Mahata, S.K., et al. ÔNovel autocrine feedback control of catecholamine release. A discrete chromogranin a fragment is a noncompetitive nicotinic cholinergic antagonist.Õ J. Clin. Invest., 100, 1623-1633 (1997).
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Test
You can block the antibody by the specific target amino acid sequence of peptide.
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Properties
blocking peptide
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Description
Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
