His-tag, 6xHis (100g)

  • Catalog number
    AS15 2937
  • Price
    Please ask
  • Size
    100 µg (1 mg/ml)
  • Available ordering format
    Liquid
  • Immunogen
    Polyhistidine (HHHHHH) tagged polypeptide
  • Raised in
    Mouse
  • Clonality
    Monoclonal
  • Clone
    Clone AD1.1.10
  • Purification
    Protein A purified
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    Store at 2-8°CShelf life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
  • Verified applications
    Flow cytometry (FL), cell-based assays (CBA), microarrays, and microplate applications
  • Connected products
    AS15 2930 | 6x Histidine, HRP conjugated (mouse antibody)AS15 2935 | 6x Histidine, ALP Conjugated (mouse antibody)AS15 2934 | 6x Histidine, Biotin Conjugated (mouse antibody)AS15 2928 | 6x Histidine, conjugated to DyLight® 488 (mouse antibody)AS15 2929 | 6x Histidine, conjugated to DyLight® 550 (mouse antibody)AS15 2933 | 6x Histidine, conjugated to DyLight® 650 (mouse antibody)AS15 2932 | 6x Histidine, conjugated to Europium (mouse antibody)AS15 2931 | 6x Histidine, conjugated to SureLight® R-Phycoerythrin (mouse antibody)other tag antibodies
  • Recommended dilutions for use
    to be determined by end user
  • Molecular weight expected аpparent
    see datasheet
  • Verified reactivity
    to be determined
  • Possible reactivity
    to be determined
  • No reactivity
    to be determined
  • Supplementary information
    Contact Gentaur support center at [email protected]
  • References
    to be added when available, antibody released in August 2015
  • Scientific context
    His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
  • Notes
    Antibody is provided in: 100 mM Sodium Phosphate pH 7.4, 100 mM NaCl, 50 mM Sucrose, 0.1% BSA and 2 mM Sodium Azide
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Conjugated
    The polyhistidine (6xHis) tag or Histidine Epitope Tag (HIS Tag, 6XHIS Tag with Histidine (abbreviated as His or H; encoded by the codons CAU and CAC) is an α-amino acid that is used in the biosynthesis of proteins. It is the most used tag for purification by Affinity Chromatography (IMAC). The pentadentate chelator is the best chelator in Immobilized Metal ion Affinity chromatography (IMAC) for the6xhis-tagged proteins.
  • Conjugation
    histidine
  • Gene target
    His-tag   100g  
  • Short name
    His-tag, (100g)
  • Technique
    Tags
  • Label
    His
  • Alternative name
    histidine-detection labelled, 6xHis (100g)
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