Hi-Bind™ Ni QR Agarose Beads

  • Catalog number
    6562-10
  • Price
    Please ask
  • Size
    10 ml
  • Description
    Reusable Ni ion conjugated agarose beads with > 60 mg/ml binding capacity & > 4cm/min flow rate for purification of metal ions binding proteins. Basic research of the physico-chemical properties as well as the activity of proteins is impossible without their isolation and purification. Majority of protein studies are carried out on tagged recombinant proteins expressed in various host organisms. Over 50% of these are expressed as fusions with poly-histidine purification tags. The small size and the mild conditions utilized during purification as well as the low cost of purification made this type of fusion the most popular (and in many cases, the first tag of choice). Hi-Bind™ Ni QR Agarose Beads combines the capacity of immobilized nickel ions with the extreme flow rates of Quick Run (QR) Agarose beads. These beads deliver as much as 25% higher capacity than Ni-NTA adsorbents while dramatically decreasing purification times due to the ability to perform washing steps at linear flow rates higher than 4 cm/min.
  • Product Highlights
    • Binding Capacity: Up to 60 mg target protein/ml of settled resin. The binding capacity for individual proteins may vary. • Content: Supplied as 50% slurry (e.g., 1 ml of settled resin is equivalent to 2 ml of 50% slurry) in 20% ethanol. • Flow Rate: Higher than 4 cm/min.
  • Storage Temp
    +4°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Notes
    For research use only. Not to be used for human or animal treatment or consumption.
  • Properties
    The purest agarose was used in the production of Hi-Bind™ Ni QR Agarose by Biovision.
  • Conjugation
    Agarose
  • Gene target
  • Short name
    Hi-Bind™ Ni QR Agarose
  • Technique
    Agarose, agaroses, All kind of bead conjugates are available, magnetic, borosilicate, agarose protein A. magnetic beads can be isolated by a macs type magnet.
  • Label
    Agarose
  • Alternative name
    Hi-Bind™ Ni QR molecular sieve particles
  • Alternative technique
    separation
MeSH Data
  • Name
  • Concept
    Scope note: Electrophoresis in which agar or agarose gel is used as the diffusion medium.
  • Tree numbers
    • E05.196.401.153
    • E05.301.300.100
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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