HHBS [Hanks' Buffer with 20 mM Hepes]

• Catalog number
  20011
• Price
  Please ask
• Size
  2X100 mL

• Description
  HHBS [Hanks Buffer with 20 mM Hepes] is a solution that is used for a variety of cell culture applications, such as washing cells, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
• Similar product
• Shipping
  Room Temperature
• Storage
  Freezer
• Shelflife
  6 months
• Molecular Weight
  NA
• Appearance
  Solid
• Solubility
  NA
• Excitation Wavelength
  NA
• Emission Wavelength
  NA
• Personal precautions
  Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
• Uses
  Laboratory chemicals *For Research Use Only*
• Technical data
• Data sheet
• Ordering
  To order HHBS [Hanks' Buffer with 20 mM Hepes], please use the Cat. Nr.20011 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
• Tips
  Our specialists recommend you to follow carefully the pre-registered instructions for HHBS [Hanks' Buffer with 20 mM Hepes]
• Additional description
  Buffering solutions are useful to keep the pH range sable when using this reagent of aat.
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.

• Gene target
  HHBS   [Hanks   with  
• Short name
  HHBS [Hanks' with 20 mM ]
• Alternative name
  HHBS [Hanks' solution including 20 millimeter Hepes]

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