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Alternative name1
Anti-HexaHistidine (Biotin)
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Alternative name2
HexaHistidine (Biotin)
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Alternative name3
HexaHistidine (Biotin)
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Alternative name4
HexaHistidine
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Alternative name5
Anti -HexaHistidine (Biotin)
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Other names
hexahistidine peptide; N/A
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Category
Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
IgY
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Clone
Polyclonal antibody
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Host organism
Chicken
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Species reactivity
N/A
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Specificity and cross reactivity
Recognizes HexaHistidine.
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Purification method
Affinity Purified by immunoaffinity chromatography.
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Form Appearance
Supplied as a liquid in 25mM HEPES, pH 7.0, 0.05M sodium chloride, 0.1% BSA, 0.05% sodium azide, 50% glycerol. Labeled with Biotin.
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Concentration
N/A
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Storage and shipping
Store at 4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, productone should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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Tested for
ELISA (EL/EIA)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
