Heregulin-B1 Recombinant Protein

• Catalog number
  40-518
• Price
  Please ask
• Size
  0.05 mg

• About recombinant protein
  Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms release soluble growth factors. HRG1-B1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation. Although HRG1-B1 biological effects is still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF). Recombinant human Heregulin-B1 (HRG1-B1) is a 7.5 kDa polypeptide consisting of only the EGF domain of heregulin-B1 (65 amino acid residues).
• Other name of the recombinant
  GGF, HGL, HRG, NDF, ARIA, GGF2, HRG1, HRGA, SMDF, MST131, GGF, Pro-neuregulin-1, membrane-bound isoform, Acetylcholine receptor-inducing activity, Pro-NRG1
• Recombinant in
  E. coli
• His tag on the recombinant protein
  no His tag
• SEQUENCE
  SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE
• Activity
  The ED50 was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is ≤ 0.5 ng/mL, corresponding to a specific activity of ≥ 2 x 106 units/mg.
• Keep at
  The lyophilized Heregulin-B1 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted GDF-2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Heregulin-B1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  236461846
• Accession nr
  NP_001153468.1
• Pureity
  Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
• About
  This product is for research use only.
• Protein s origin
  Human
• Vial
  Lyophilized
• NCBI ID
  3084
• Gene name
  neuregulin 1
• Source
  Homo sapiens
• Uniprot
  Q02297
• Pictures of recombinant
• Rec pdf
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Heregulin-B1   Protein  
• Gene symbol
  NRG1
• Short name
  Heregulin-B1 Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  Heregulin-B1 Rec. Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:43633
• Gene
  NRG1-IT1
• Long gene name
  NRG1 intronic transcript 1
• Synonyms gene name
  • NRG1 intronic transcript 1 (non-protein coding)
• GenBank acession
  • BC037250
• Locus
  8p12
• Discovery year
  2011-12-06
• Classification
  • Intronic transcripts
• VEGA ID
  OTTHUMG00000163966

Gene info

• Identity
  HGNC:7997
• Gene
  NRG1
• Long gene name
  neuregulin 1
• Synonyms gene
  • HGL
  • NRG1-IT2
• Synonyms gene name
  • NRG1 intronic transcript 2 (non-protein coding)
• Synonyms
  • HRG
  • NDF
  • GGF
• GenBank acession
  • L12261
• Locus
  8p12
• Discovery year
  1999-03-19
• Entrez gene record
  3084
• Pubmed identfication
  • 1350381
  • 8095334
• Classification
  • I-set domain containing
  • Neuregulins
• VEGA ID
  OTTHUMG00000163918

Gene info

• Identity
  HGNC:43635
• Gene
  NRG1-IT3
• Long gene name
  NRG1 intronic transcript 3
• Synonyms gene name
  • NRG1 intronic transcript 3 (non-protein coding)
• Locus
  8p12
• Discovery year
  2011-12-06
• Classification
  • Intronic transcripts
• VEGA ID
  OTTHUMG00000164003

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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