HEPES Sodium salt Ultrapure
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Catalog number
63-82898105
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Price
Please ask
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Size
100 g
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CAS number
[75277-39-3]
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Availability
Please contact Gentaur/Genprice to check the latest availability
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Ordering
For ordering the HEPES Sodium salt Ultrapure please use the Catalogue ID#82898105 in your purchase order among with the desired quantity, your shipping and billing addresses, as well applicable VAT number. Please contact Gentaur for bulk or larger quantity, a discount may apply.
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Technical files
Please contact our technical support to request the latest datasheet, certificate of analysis or the material safety datasheet
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Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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