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Chemical grade
Biotechnology
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CAS number
75277-39-3
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Storage requirements
Store at room temperature. Protect from moisture
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Category
Biochemicals
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Hazardous number
Please see datasheet or contact us for more information and details about hazardous materials.
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Chemical class
Please see datasheet
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Chemical PG
No Data Available
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Supplementary technical information
Contact us for more technical information
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Notes
For research use only. Not for diagnostic procedures.
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Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
